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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SERPINE2 Recombinant antibody (82732-5-RR)

    WB analysis using 82732-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82732-5-RR (SERPINE2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using SERPINE2 Recombinant antibody (82732-5-RR)

    WB analysis using 82732-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82732-5-RR (SERPINE2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of A549 cells using SERPINE2 Recombinant antibody (82732-5-RR)

    FC experiment of A549 using 82732-5-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug SERPINE2 Recombinant antibody (82732-5-RR, Clone:5O16) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 82732-5-RR

    Affinity and Kinetic Characterization of 82732-5-RR

    Biolayer interferometry (BLl) kinetic assays of 82732-5-RR against Human SERPINE2 were performed. The affinity constant is below 1 pM.

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SERPINE2 Recombinant antibody

SERPINE2 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 82732-5-RR
Clone No.5O16

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Synonyms

PI7, PN1, Serpin E2, PI 7, Peptidase inhibitor 7

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inU-87 MG cells, C6 cells, U-251 cells, mouse brain tissue, rat brain tissue
Positive FC (Intra) detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82732-5-RR targets SERPINE2 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SERPINE2 fusion protein Ag1830 相同性解析による交差性が予測される生物種
Full Name serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 2
Calculated molecular weight 44 kDa
Observed molecular weight 44-50 kDa
GenBank accession numberBC015663
Gene Symbol SERPINE2
Gene ID (NCBI) 5270
RRIDAB_3670540
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP07093
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SERPINE2 also called protease nexin-1 or glial-derived neurite promoting factor, is a member of the serine protease inhibitor (SERPIN) superfamily. It would be a potent inhibitor of the urokinase-plasminogen activator (uPA), tissue-type PA (tPA), thrombin, trypsin, factor Xia, and prostasin. SERPINE2 is widely expressed in various tissues, including endothelial cells, fibroblasts, smooth muscle cells, tumor cells, glial cells, neurons, and placental cells.

Protocols

Product Specific Protocols
WB protocol for SERPINE2 antibody 82732-5-RRDownload protocol
FC protocol for SERPINE2 antibody 82732-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols