SERPING1/C1 Inactivator Polyclonal antibody

Name: SERPING1/C1 Inactivator Polyclonal antibody
Brand: Proteintech
SKU: 12259-1-AP

SERPING1/C1 Inactivator Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 12259-1-AP

Synonyms

Serpin G1, SERPING1, C1NH, C1-inhibiting factor, C1IN

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis using 12259-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12259-1-AP (SERPING1/C1 Inactivator antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of L02 using 12259-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 12259-1-AP (SERPING1/C1 Inactivator antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 12259-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 12259-1-AP (SERPING1/C1 Inactivator antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 12259-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 12259-1-AP (SERPING1/C1 Inactivator antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 12259-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 12259-1-AP (SERPING1/C1 Inactivator antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 12259-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 12259-1-AP (SERPING1/C1 Inactivator antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 12259-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 12259-1-AP (SERPING1/C1 Inactivator antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of human tonsillitis using 12259-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12259-1-AP (SERPING1/C1 Inactivator antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 12259-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12259-1-AP (SERPING1/C1 Inactivator antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human normal colon using 12259-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 12259-1-AP (SERPING1/C1 Inactivator antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human normal colon using 12259-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 12259-1-AP (SERPING1/C1 Inactivator antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 12259-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SERPING1/C1 Inactivator antibody (12259-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of HepG2 using 12259-1-AP
Immunofluorescent analysis of HepG2 cells using 12259-1-AP (SERPING1/C1 Inactivator antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 12259-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	human plasma, HeLa cells, human liver tissue, L02 cells, mouse liver tissue, HepG2 cells, human urine tissue
Positive IHC detected in	human tonsillitis tissue, human normal colon **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:200-1:800
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 5 publications below
IHC	See 3 publications below
IF	See 3 publications below

Product Information

12259-1-AP targets SERPING1/C1 Inactivator in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SERPING1/C1 Inactivator fusion protein Ag2819 相同性解析による交差性が予測される生物種
Full Name	serpin peptidase inhibitor, clade G (C1 inhibitor), member 1
Calculated molecular weight	500 aa, 55 kDa
Observed molecular weight	100 kDa
GenBank accession number	BC011171
Gene symbol	SERPING1
Gene ID (NCBI)	710
RRID	AB_2285663
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SERPING1, also known as C1 Inhibitor (C1INH), is a member of the Serine proteinase inhibitor family. Its main function is the inhibition of the complement system to prevent spontaneous activation. It inhibits activated C1r and C1s of the first complement component and thus regulates complement activation. Deficiency of this protein is associated with hereditary angioneurotic oedema (HANE). C1INH is the most heavily glycosylated plasma protein. Of its 95-105 kDa apparent molecular mass, the protein moiety of 500 aa accounts for only 55 kDa. The native 105 kDa form of C1INH could be cleaved into 60-65 kDa fragments.

Protocols

Product Specific Protocols
WB protocol for SERPING1/C1 Inactivator antibody 12259-1-AP	Download protocol
IHC protocol for SERPING1/C1 Inactivator antibody 12259-1-AP	Download protocol
IF protocol for SERPING1/C1 Inactivator antibody 12259-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Glia Knockdown of circulating C1 inhibitor induces neurovascular impairment, glial cell activation, neuroinflammation, and behavioral deficits. Authors - Dorit Farfara View Article
mouse	IF	J Neuroinflammation Fluoxetine inhibited the activation of A1 reactive astrocyte in a mouse model of major depressive disorder through astrocytic 5-HT2BR/β-arrestin2 pathway. Authors - Yinquan Fang View Article
human	WB	J Thromb Haemost The Alzheimer's disease peptide Aβ promotes thrombin generation through activation of coagulation factor XII. Authors - Daria Zamolodchikov View Article
mouse	WB	J Thromb Haemost The Alzheimer's disease peptide β-amyloid promotes thrombin generation through activation of coagulation factor XII: reply. Authors - D Zamolodchikov View Article
human	IHC	J Alzheimers Dis A New Serum Biomarker Set to Detect Mild Cognitive Impairment and Alzheimer's Disease by Peptidome Technology. Authors - Koji Abe View Article
human	IHC	Urol Oncol Decreased expression of serine protease inhibitor family G1 (SERPING1) in prostate cancer can help distinguish high-risk prostate cancer and predicts malignant progression. Authors - Shengmeng Peng View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SERPING1/C1 Inactivator Polyclonal antibody

SERPING1/C1 Inactivator Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis using 12259-1-AP

WB analysis of L02 using 12259-1-AP

WB analysis of mouse liver using 12259-1-AP

WB analysis of HeLa using 12259-1-AP

WB analysis of human liver using 12259-1-AP

WB analysis of HepG2 using 12259-1-AP

WB analysis of mouse liver using 12259-1-AP

IHC staining of human tonsillitis using 12259-1-AP

IHC staining of human tonsillitis using 12259-1-AP

IHC staining of human normal colon using 12259-1-AP

IHC staining of human normal colon using 12259-1-AP

IF Staining of HepG2 using 12259-1-AP

IF Staining of HepG2 using 12259-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Knockdown of circulating C1 inhibitor induces neurovascular impairment, glial cell activation, neuroinflammation, and behavioral deficits.

Fluoxetine inhibited the activation of A1 reactive astrocyte in a mouse model of major depressive disorder through astrocytic 5-HT2BR/β-arrestin2 pathway.

The Alzheimer's disease peptide Aβ promotes thrombin generation through activation of coagulation factor XII.

The Alzheimer's disease peptide β-amyloid promotes thrombin generation through activation of coagulation factor XII: reply.

A New Serum Biomarker Set to Detect Mild Cognitive Impairment and Alzheimer's Disease by Peptidome Technology.

Decreased expression of serine protease inhibitor family G1 (SERPING1) in prostate cancer can help distinguish high-risk prostate cancer and predicts malignant progression.