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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SESN3 Recombinant antibody, PBS Only

SESN3 Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

242189D1

Cat no : 84823-3-PBS

Synonyms

Sestrin-3, sestrin 3, SEST3, EC:1.11.1.-, 242189D1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SESN3 Recombinant antibody (84823-3-RR)

    WB analysis using 84823-3-RR (same clone as 84823-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84823-3-RR (SESN3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84823-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using SESN3 Recombinant antibody (84823-3-RR)

    IHC staining of mouse liver using 84823-3-RR (same clone as 84823-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84823-3-RR (SESN3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84823-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SESN3 Recombinant antibody (84823-3-RR)

    IF Staining of HepG2 using 84823-3-RR (same clone as 84823-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SESN3 antibody (84823-3-RR, Clone: 242189D1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84823-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84823-3-RR

    Affinity and Kinetic Characterization of 84823-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84823-3-RR against Human SESN3 were performed. The affinity constant is 6.78 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84823-3-PBS targets SESN3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SESN3 fusion protein Ag17872 相同性解析による交差性が予測される生物種
Full Name sestrin 3
Calculated molecular weight 492 aa, 57 kDa
Observed molecular weight57-60 kDa
GenBank accession numberBC017296
Gene symbol SESN3
Gene ID (NCBI) 143686
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Sestrins, including sestrin-1 (PA26), sestrin-2 (Hi95), and sestrin-3, are 48 to 65 kDa cystein sulfinyl reductases and modulate peroxide signaling and antioxidant defense. These proteins selectively reduce or repair hyperoxidized forms of typical 2-Cys peroxiredoxins within eukaryotes. Expression of these proteins is regulated by p53, a tumor suppressor protein. Sestrin 3 was identified as a forkhead box O (FoxO) target gene with antioxidant activity. Recently it was reported that sestrin 3 may play an important role in AKT-induced increase in ROS. It might be a promising target in selectively killing cancer cells with high AKT activity levels.