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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SESN3 Recombinant antibody

SESN3 Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

242189D1

Cat no : 84823-3-RR

Synonyms

Sestrin-3, sestrin 3, SEST3, EC:1.11.1.-, 242189D1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SESN3 Recombinant antibody (84823-3-RR)

    WB analysis using 84823-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84823-3-RR (SESN3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using SESN3 Recombinant antibody (84823-3-RR)

    IHC staining of mouse liver using 84823-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84823-3-RR (SESN3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SESN3 Recombinant antibody (84823-3-RR)

    IF Staining of HepG2 using 84823-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SESN3 antibody (84823-3-RR, Clone: 242189D1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84823-3-RR

    Affinity and Kinetic Characterization of 84823-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84823-3-RR against Human SESN3 were performed. The affinity constant is 6.78 nM.

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Tested Applications

Positive WB detected inA549 cells, A431 cells, HepG2 cells, HEK-293 cells
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84823-3-RR targets SESN3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SESN3 fusion protein Ag17872 相同性解析による交差性が予測される生物種
Full Name sestrin 3
Calculated molecular weight 492 aa, 57 kDa
Observed molecular weight57-60 kDa
GenBank accession numberBC017296
Gene symbol SESN3
Gene ID (NCBI) 143686
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Sestrins, including sestrin-1 (PA26), sestrin-2 (Hi95), and sestrin-3, are 48 to 65 kDa cystein sulfinyl reductases and modulate peroxide signaling and antioxidant defense. These proteins selectively reduce or repair hyperoxidized forms of typical 2-Cys peroxiredoxins within eukaryotes. Expression of these proteins is regulated by p53, a tumor suppressor protein. Sestrin 3 was identified as a forkhead box O (FoxO) target gene with antioxidant activity. Recently it was reported that sestrin 3 may play an important role in AKT-induced increase in ROS. It might be a promising target in selectively killing cancer cells with high AKT activity levels.

Protocols

Product Specific Protocols
WB protocol for SESN3 antibody 84823-3-RRDownload protocol
IHC protocol for SESN3 antibody 84823-3-RRDownload protocol
IF protocol for SESN3 antibody 84823-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols