CoraLite®594-conjugated SF3B3 Polyclonal antibody
SF3B3 Polyclonal Antibody for IF-P
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF-P
Conjugate
CoraLite®594 Fluorescent Dye
Cat no : CL594-14577
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF-P detected in | mouse heart tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL594-14577 targets SF3B3 in IF-P applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SF3B3 fusion protein Ag5980 相同性解析による交差性が予測される生物種 |
| Full Name | splicing factor 3b, subunit 3, 130kDa |
| Calculated molecular weight | 136 kDa |
| Observed molecular weight | 130-135 kDa |
| GenBank accession number | BC003146 |
| Gene symbol | SF3B3 |
| Gene ID (NCBI) | 23450 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Introns are removed from nuclear pre-mRNA in 2-step transesterification reactions. Splicing occured in a large ribonucleoprotein particle, called the spliceosome. Spliceosomal intermediate complexes form on pre-mRNA in the order E, A, B, and C, with the catalytic reactions occurring in complex C. U2 small nuclear ribonucleoproteins are one of the proteins essential for spliceosome assembly and mRNA splicing. Functional U2 snRNP is composed of a 12S unit and 2 splicing factors, SF3A, which is composed of 3 proteins, and SF3B, which conposed of 4 proteins. SF3B3 is one of SF3B, and it's required for 'A' complex assembly formed by the stable binding of U2 snRNP to the brachpoint sequence(BPS) in pre-mRNA.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 SF3B3 antibody CL594-14577 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
