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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SFRP2 Monoclonal antibody, PBS Only

SFRP2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig, rabbit, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F3A1

Cat no : 66328-1-PBS

Synonyms

FRP 2, FRP2, SARP 1, SARP1, SDF 5, sFRP 2, SFRP2, UNQ361/PRO697

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using SFRP2 Monoclonal antibody (66328-1-Ig)

    WB analysis using 66328-1-Ig (same clone as 66328-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66328-1-Ig (SFRP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66328-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using SFRP2 Monoclonal antibody (66328-1-Ig)

    WB analysis of rat brain using 66328-1-Ig (same clone as 66328-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 66328-1-Ig (SFRP2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66328-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Y79 cells using SFRP2 Monoclonal antibody (66328-1-Ig)

    WB analysis of Y79 using 66328-1-Ig (same clone as 66328-1-PBS)

    Y79 cells were subjected to SDS PAGE followed by western blot with 66328-1-Ig (SFRP2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66328-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COLO 320 cells using SFRP2 Monoclonal antibody (66328-1-Ig)

    WB analysis of COLO 320 using 66328-1-Ig (same clone as 66328-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 66328-1-Ig (SFRP2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66328-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human malignant melanoma tissue using SFRP2 Monoclonal antibody (66328-1-Ig)

    IHC staining of human malignant melanoma using 66328-1-Ig (same clone as 66328-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 66328-1-Ig (SFRP2 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66328-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human malignant melanoma tissue using SFRP2 Monoclonal antibody (66328-1-Ig)

    IHC staining of human malignant melanoma using 66328-1-Ig (same clone as 66328-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 66328-1-Ig (SFRP2 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66328-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using SFRP2 Monoclonal antibody (66328-1-Ig)

    IF Staining of A549 using 66328-1-Ig (same clone as 66328-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using SFRP2 antibody (66328-1-Ig, Clone: 1F3A1 ) at dilution of 1:1500 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66328-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66328-1-PBS targets SFRP2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat, pig, rabbit, mouse samples.

Tested Reactivity human, rat, pig, rabbit, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SFRP2 fusion protein Ag18840 相同性解析による交差性が予測される生物種
Full Name secreted frizzled-related protein 2
Calculated molecular weight 295 aa, 34 kDa
Observed molecular weight 38 kDa
GenBank accession numberBC008666
Gene symbol SFRP2
Gene ID (NCBI) 6423
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Secreted frizzled-related protein 2 (Sfrp2) is a secreted glycoprotein molecule containing an N terminal cysteine-rich domain, which is typically 30-50% identical to the putative Wnt-binding site of the frizzled receptor, and a C terminal heparin-binding domain with weak homology to netrins. It has been implicated in diverse cellular processes, including embryogenesis, regulation of cell apoptosis, and cell differentiation. Methylation of this gene is a potential marker for the presence of colorectal cancer.