SFRP2 Monoclonal antibody
SFRP2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, rat, pig, rabbit, mouse
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
1F3A1
Cat no : 66328-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | pig brain tissue, Y79 cells, COLO 320 cells, rat brain tissue, rabbit brain tissue, mouse brain tissue |
| Positive IHC detected in | human malignant melanoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:750-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
66328-1-Ig targets SFRP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat, pig, rabbit, mouse samples.
| Tested Reactivity | human, rat, pig, rabbit, mouse |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | SFRP2 fusion protein Ag18840 相同性解析による交差性が予測される生物種 |
| Full Name | secreted frizzled-related protein 2 |
| Calculated molecular weight | 295 aa, 34 kDa |
| Observed molecular weight | 38 kDa |
| GenBank accession number | BC008666 |
| Gene symbol | SFRP2 |
| Gene ID (NCBI) | 6423 |
| RRID | AB_2881709 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Secreted frizzled-related protein 2 (Sfrp2) is a secreted glycoprotein molecule containing an N terminal cysteine-rich domain, which is typically 30-50% identical to the putative Wnt-binding site of the frizzled receptor, and a C terminal heparin-binding domain with weak homology to netrins. It has been implicated in diverse cellular processes, including embryogenesis, regulation of cell apoptosis, and cell differentiation. Methylation of this gene is a potential marker for the presence of colorectal cancer.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SFRP2 antibody 66328-1-Ig | Download protocol |
| IHC protocol for SFRP2 antibody 66328-1-Ig | Download protocol |
| IF protocol for SFRP2 antibody 66328-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Exp Cell Res miR-629-5p promotes growth and metastasis of hepatocellular carcinoma by activating β-catenin. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Kenzo (Verified Customer) (08-30-2023) | This antibody worked well for mouse tissues when used at 1:200 and incubated for overnight.
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