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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated SFRP2 Monoclonal antibody

SFRP2 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1F3A1

Cat no : CL488-66328

Synonyms

FRP 2, FRP2, SARP 1, SARP1, SDF 5, sFRP 2, SFRP2, UNQ361/PRO697

Validation Data Gallery

Filter:
  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using CoraLite® Plus 488-conjugated SFRP2 Monoclonal ant (CL488-66328)

    IF Staining of SH-SY5Y using CL488-66328

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using CL488-66328 (SFRP2 antibody) at dilution of 1:100.

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Tested Applications

Positive IF/ICC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66328 targets SFRP2 in IF/ICC applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SFRP2 fusion protein Ag18840 相同性解析による交差性が予測される生物種
Full Name secreted frizzled-related protein 2
Calculated molecular weight 295 aa, 34 kDa
Observed molecular weight 38 kDa
GenBank accession numberBC008666
Gene symbol SFRP2
Gene ID (NCBI) 6423
RRIDAB_2883310
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Secreted frizzled-related protein 2 (Sfrp2) is a secreted glycoprotein molecule containing an N terminal cysteine-rich domain, which is typically 30-50% identical to the putative Wnt-binding site of the frizzled receptor, and a C terminal heparin-binding domain with weak homology to netrins. It has been implicated in diverse cellular processes, including embryogenesis, regulation of cell apoptosis, and cell differentiation. Methylation of this gene is a potential marker for the presence of colorectal cancer.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 SFRP2 antibody CL488-66328Download protocol
Standard Protocols
Click here to view our Standard Protocols