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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SGLT2 Polyclonal antibody, PBS Only

SGLT2 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

Cat no : 28683-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SGLT2 Polyclonal antibody (28683-1-AP)

    WB analysis using 28683-1-AP (same clone as 28683-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28683-1-AP (SGLT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28683-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using SGLT2 Polyclonal antibody (28683-1-AP)

    IHC staining of human kidney using 28683-1-AP (same clone as 28683-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28683-1-AP (SGLT2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28683-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using SGLT2 Polyclonal antibody (28683-1-AP)

    IHC staining of mouse kidney using 28683-1-AP (same clone as 28683-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 28683-1-AP (SGLT2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28683-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse kidney tissue using SGLT2 Polyclonal antibody (28683-1-AP)

    IF Staining of mouse kidney using 28683-1-AP (same clone as 28683-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using SGLT2 antibody (28683-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 28683-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat kidney tissue using SGLT2 Polyclonal antibody (28683-1-AP)

    IF Staining of rat kidney using 28683-1-AP (same clone as 28683-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed rat kidney tissue using SGLT2 antibody (28683-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 28683-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

28683-1-PBS targets SGLT2 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SGLT2 fusion protein Ag30120 相同性解析による交差性が予測される生物種
Full Name solute carrier family 5 (sodium/glucose cotransporter), member 2
Calculated molecular weight 672 aa, 73 kDa
Observed molecular weight73 kDa
GenBank accession numberBC131542
Gene symbol SGLT2
Gene ID (NCBI) 6524
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Sodium-glucose cotransporter 2 (SGLT2), encoded by SLC5A2, utilizes the electrochemical sodium gradient to transport glucose against the cell's internal concentration gradient. Mainly expressed on brush border membrane (BBM) of epithelial cells in the early segment of the proximal tubule, SGLT2 mediates most of the glucose reabsorption by the kidney overall. Inhibition of SGLT2 could improve glucose homeostasis of diabetic patients, which has been considered as a novel strategy for diabetes treatment.