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Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using SGTA Polyclonal antibody (11019-2-AP)

    WB analysis of K-562 using 11019-2-AP (same clone as 11019-2-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 11019-2-AP (SGTA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11019-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using SGTA Polyclonal antibody (11019-2-AP)

    WB analysis of mouse liver using 11019-2-AP (same clone as 11019-2-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 11019-2-AP (SGTA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11019-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SH-SY5Y cells using SGTA Polyclonal antibody (11019-2-AP)

    WB analysis of SH-SY5Y using 11019-2-AP (same clone as 11019-2-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 11019-2-AP (SGTA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11019-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human skeletal muscle tissue using SGTA Polyclonal antibody (11019-2-AP)

    WB analysis of human skeletal muscle using 11019-2-AP (same clone as 11019-2-PBS)

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11019-2-AP (SGTA antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11019-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using SGTA Polyclonal antibody (11019-2-AP)

    WB analysis of HeLa using 11019-2-AP (same clone as 11019-2-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 11019-2-AP (SGTA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11019-2-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using SGTA Polyclonal antibody (11019-2-AP)

    IP experiment of HeLa using 11019-2-AP (same clone as 11019-2-PBS)

    IP result of anti-SGTA (IP:11019-2-AP, 4ug; Detection:11019-2-AP 1:1000) with HeLa cells lysate 2480ug. This data was developed using the same antibody clone with 11019-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using SGTA Polyclonal antibody (11019-2-AP)

    IHC staining of human lung cancer using 11019-2-AP (same clone as 11019-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 11019-2-AP (SGTA antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11019-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SGTA Polyclonal antibody (11019-2-AP)

    IHC staining of human liver cancer using 11019-2-AP (same clone as 11019-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11019-2-AP (SGTA Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11019-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SGTA Polyclonal antibody (11019-2-AP)

    IHC staining of human liver cancer using 11019-2-AP (same clone as 11019-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11019-2-AP (SGTA Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11019-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using SGTA Polyclonal antibody (11019-2-AP)

    IHC staining of human prostate cancer using 11019-2-AP (same clone as 11019-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11019-2-AP (SGTA Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11019-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using SGTA Polyclonal antibody (11019-2-AP)

    IHC staining of human prostate cancer using 11019-2-AP (same clone as 11019-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11019-2-AP (SGTA Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11019-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SGTA Polyclonal antibody (11019-2-AP)

    IF Staining of HeLa using 11019-2-AP (same clone as 11019-2-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 11019-2-AP (SGTA antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11019-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SGTA Polyclonal antibody (11019-2-AP)

    IF Staining of HeLa using 11019-2-AP (same clone as 11019-2-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using SGTA antibody (11019-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 11019-2-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

SGTA Polyclonal antibody, PBS Only

SGTA Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 11019-2-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11019-2-PBS targets SGTA in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SGTA fusion protein Ag1475 相同性解析による交差性が予測される生物種
Full Name small glutamine-rich tetratricopeptide repeat (TPR)-containing, alpha
Calculated molecular weight 34 kDa
Observed molecular weight 34-37 kDa
GenBank accession numberBC008885
Gene Symbol SGTA
Gene ID (NCBI) 6449
RRIDAB_2188830
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43765
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Small glutamine-rich tetratricopeptide repeat-containing protein alpha (SGTA) is a steroid receptor molecular co-chaperone critical in a range of biological processes, including viral infection, cell division, mitosis, and cell cycle checkpoint activation. Overexpression of SGTA has been found in various cancers, including prostate/ovary/testis/liver/lung cancer.