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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse thymus tissue using SH2D1A Polyclonal antibody (11868-1-AP)

    WB analysis of mouse thymus using 11868-1-AP (same clone as 11868-1-PBS)

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11868-1-AP (SH2D1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11868-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using SH2D1A Polyclonal antibody (11868-1-AP)

    WB analysis of Jurkat using 11868-1-AP (same clone as 11868-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 11868-1-AP (SH2D1A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11868-1-PBS in a different storage buffer formulation.

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SH2D1A Polyclonal antibody, PBS Only

SH2D1A Polyclonal Antibody for WB, Indirect ELISA
Cat No. 11868-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11868-1-PBS targets SH2D1A in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SH2D1A fusion protein Ag2450 相同性解析による交差性が予測される生物種
Full Name SH2 domain protein 1A
Calculated molecular weight 128 aa, 14 kDa
Observed molecular weight 16 kDa
GenBank accession numberBC020732
Gene Symbol SH2D1A
Gene ID (NCBI) 4068
RRIDAB_2239283
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60880
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

SH2D1A, also named as DSHP and SAP, is an inhibitor of the SLAM self-association. It acts by blocking recruitment of the SH2-domain-containing signal-transduction molecule SHP-2 to a docking site in the SLAM cytoplasmic region. SH2D1A mediates interaction between FYN and SLAMF1. Defects in SH2D1A are a cause of lymphoproliferative syndrome X-linked type 1 (XLP1).