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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 750-conjugated SHBG Recombinant antibody

SHBG Recombinant Antibody for WB

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

230356F10

Cat no : CL750-83096

Synonyms

ABP, SBP, sex hormone binding globulin, Sex steroid binding protein, SHBG, TEBG

Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using CoraLite® Plus 750-conjugated SHBG Recombinant ant (CL750-83096)

    WB analysis of human plasma using CL750-83096

    Various lysates were subjected to SDS PAGE followed by western blot with CL750-83096 (SHBG antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inhuman plasma

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL750-83096 targets SHBG in WB applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name sex hormone-binding globulin
Calculated molecular weight 402 aa, 44 kDa
Observed molecular weight40-50 kDa
GenBank accession numberBC101785
Gene symbol SHBG
Gene ID (NCBI) 6462
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission maxima wavelengths755 nm / 780 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SHBG (sex hormone-binding globulin), also known as androgen-binding protein (ABP), is the specific transport protein for sex steroid hormones in humans. SHBG is synthesized mainly by the liver and circulates in the blood, in form of homodimer with a single steroid-binding site per dimer. In addition to the plasma SHBG, a testis isoform expressed by Sortoli cells also exists due to the gene alternative splicing. Various bands (44-56 kDa) recognized by this antibody may represent variably glycosylated or different isoforms of SHBG.

Protocols

Product Specific Protocols
WB protocol for CL Plus 750 SHBG antibody CL750-83096Download protocol
Standard Protocols
Click here to view our Standard Protocols