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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SHMT1 Monoclonal antibody

SHMT1 Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig, rabbit

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2D5E6

Cat no : 67963-1-Ig

Synonyms

CSHMT, Serine methylase, SHMT, SHMT1

Validation Data Gallery

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  • Western Blot (WB) analysis of rabbit liver tissue using SHMT1 Monoclonal antibody (67963-1-Ig)

    WB analysis of rabbit liver using 67963-1-Ig

    rabbit liver tissue were subjected to SDS PAGE followed by western blot with 67963-1-Ig (SHMT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using SHMT1 Monoclonal antibody (67963-1-Ig)

    WB analysis using 67963-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67963-1-Ig (SHMT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SHMT1 Monoclonal antibody (67963-1-Ig)

    IF Staining of HepG2 using 67963-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using SHMT1 antibody (67963-1-Ig, Clone: 2D5E6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, rabbit liver tissue, HepG2 cells, Jurkat cells, K-562 cells, Pig liver tissue
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67963-1-Ig targets SHMT1 in WB, IF, ELISA applications and shows reactivity with Human, pig, rabbit samples.

Tested Reactivity Human, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SHMT1 fusion protein Ag5833 相同性解析による交差性が予測される生物種
Full Name serine hydroxymethyltransferase 1 (soluble)
Calculated molecular weight 483 aa, 53 kDa
Observed molecular weight45-53 kDa
GenBank accession numberBC038598
Gene symbol SHMT1
Gene ID (NCBI) 6470
RRIDAB_2918714
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SHMT1 antibody 67963-1-IgDownload protocol
IF protocol for SHMT1 antibody 67963-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols