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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SHMT1 Monoclonal antibody, PBS Only

SHMT1 Monoclonal Antibody for WB, IF, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig, rabbit

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D5E6

Cat no : 67963-1-PBS

Synonyms

CSHMT, Serine methylase, SHMT, SHMT1

Validation Data Gallery

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  • Western Blot (WB) analysis of rabbit liver tissue using SHMT1 Monoclonal antibody (67963-1-Ig)

    WB analysis of rabbit liver using 67963-1-Ig (same clone as 67963-1-PBS)

    rabbit liver tissue were subjected to SDS PAGE followed by western blot with 67963-1-Ig (SHMT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67963-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using SHMT1 Monoclonal antibody (67963-1-Ig)

    WB analysis using 67963-1-Ig (same clone as 67963-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67963-1-Ig (SHMT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67963-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SHMT1 Monoclonal antibody (67963-1-Ig)

    IF Staining of HepG2 using 67963-1-Ig (same clone as 67963-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using SHMT1 antibody (67963-1-Ig, Clone: 2D5E6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67963-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67963-1-PBS targets SHMT1 in WB, IF, Indirect ELISA applications and shows reactivity with Human, pig, rabbit samples.

Tested Reactivity Human, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SHMT1 fusion protein Ag5833 相同性解析による交差性が予測される生物種
Full Name serine hydroxymethyltransferase 1 (soluble)
Calculated molecular weight 483 aa, 53 kDa
Observed molecular weight45-53 kDa
GenBank accession numberBC038598
Gene symbol SHMT1
Gene ID (NCBI) 6470
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.