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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SHMT2 Monoclonal antibody, PBS Only

SHMT2 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3A2H9

Cat no : 67980-1-PBS

Synonyms

GLYA, Serine methylase, SHMT, SHMT2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SHMT2 Monoclonal antibody (67980-1-Ig)

    WB analysis using 67980-1-Ig (same clone as 67980-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67980-1-Ig (SHMT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67980-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using SHMT2 Monoclonal antibody (67980-1-Ig)

    WB analysis of HSC-T6 using 67980-1-Ig (same clone as 67980-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67980-1-Ig (SHMT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67980-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SHMT2 Monoclonal antibody (67980-1-Ig)

    IHC staining of human colon cancer using 67980-1-Ig (same clone as 67980-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67980-1-Ig (SHMT2 antibody) at dilution of 1:1300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67980-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SHMT2 Monoclonal antibody (67980-1-Ig)

    IHC staining of human breast cancer using 67980-1-Ig (same clone as 67980-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67980-1-Ig (SHMT2 antibody) at dilution of 1:1300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67980-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67980-1-PBS targets SHMT2 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SHMT2 fusion protein Ag1584 相同性解析による交差性が予測される生物種
Full Name serine hydroxymethyltransferase 2 (mitochondrial)
Calculated molecular weight 55 kDa
Observed molecular weight52-55 kDa
GenBank accession numberBC011911
Gene symbol SHMT2
Gene ID (NCBI) 6472
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.