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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SHMT2 Monoclonal antibody

SHMT2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3A2H9

Cat no : 67980-1-Ig

Synonyms

GLYA, Serine methylase, SHMT, SHMT2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SHMT2 Monoclonal antibody (67980-1-Ig)

    WB analysis using 67980-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67980-1-Ig (SHMT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of HSC-T6 cells using SHMT2 Monoclonal antibody (67980-1-Ig)

    WB analysis of HSC-T6 using 67980-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67980-1-Ig (SHMT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SHMT2 Monoclonal antibody (67980-1-Ig)

    IHC staining of human colon cancer using 67980-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67980-1-Ig (SHMT2 antibody) at dilution of 1:1300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SHMT2 Monoclonal antibody (67980-1-Ig)

    IHC staining of human breast cancer using 67980-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67980-1-Ig (SHMT2 antibody) at dilution of 1:1300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inNCCIT cells, HSC-T6 cells, LNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman colon cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:650-1:2600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67980-1-Ig targets SHMT2 in WB, IHC, ELISA applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SHMT2 fusion protein Ag1584 相同性解析による交差性が予測される生物種
Full Name serine hydroxymethyltransferase 2 (mitochondrial)
Calculated molecular weight 55 kDa
Observed molecular weight52-55 kDa
GenBank accession numberBC011911
Gene symbol SHMT2
Gene ID (NCBI) 6472
RRIDAB_2918729
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SHMT2 antibody 67980-1-IgDownload protocol
IHC protocol for SHMT2 antibody 67980-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols