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Validation Data Gallery

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  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 488-conjugated SHMT2 Monoclonal ant (CL488-67980)

    FC experiment of HepG2 using CL488-67980

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human SHMT2 (CL488-67980, Clone:3A2H9) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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CoraLite® Plus 488-conjugated SHMT2 Monoclonal antibody

SHMT2 Monoclonal Antibody for FC (Intra)
Cat No. CL488-67980
Clone No.3A2H9

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat

Applications

FC (Intra)

Synonyms

SHMT, EC:2.1.2.1, 3A2H9

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive FC (Intra) detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67980 targets SHMT2 in FC (Intra) applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SHMT2 fusion protein Ag1584 相同性解析による交差性が予測される生物種
Full Name serine hydroxymethyltransferase 2 (mitochondrial)
Calculated molecular weight 55 kDa
Observed molecular weight52-55 kDa
GenBank accession numberBC011911
Gene Symbol SHMT2
Gene ID (NCBI) 6472
RRIDAB_2923847
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP34897
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 SHMT2 antibody CL488-67980Download protocol
Standard Protocols
Click here to view our Standard Protocols