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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SHPK Polyclonal antibody (12441-1-AP)

    WB analysis using 12441-1-AP (same clone as 12441-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12441-1-AP (SHPK antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12441-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using SHPK Polyclonal antibody (12441-1-AP)

    WB analysis using 12441-1-AP (same clone as 12441-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12441-1-AP (SHPK antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12441-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using SHPK Polyclonal antibody (12441-1-AP)

    IHC staining of human prostate cancer using 12441-1-AP (same clone as 12441-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12441-1-AP (SHPK antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12441-1-PBS in a different storage buffer formulation.

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SHPK Polyclonal antibody, PBS Only

SHPK Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 12441-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12441-1-PBS targets SHPK in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SHPK fusion protein Ag3150 相同性解析による交差性が予測される生物種
Full Name sedoheptulokinase
Calculated molecular weight 478 aa, 52 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC020543
Gene Symbol SHPK
Gene ID (NCBI) 23729
RRIDAB_2188566
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UHJ6
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

SHPK, also named CARKL, is a carbohydrate kinase involved in the phosphorylation of sugars as they enter a cell, inhibiting return across the cell membrane (PubMed: 10673275). SHPK is a sedoheptulose kinase of the pentose phosphate pathway. It acts as a modulator of macrophage activation through control of glucose metabolism (PMID: 22682222).