• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated SHPK Recombinant antibody

SHPK Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230115A4

Cat no : CL488-82875

Synonyms

SHK, Sedoheptulokinase, EC:2.7.1.14, CARKL, Carbohydrate kinase-like protein

Validation Data Gallery

Filter:
  • Flow cytometry (FC) experiment of U2OS cells using CoraLite® Plus 488-conjugated SHPK Recombinant ant (CL488-82875)

    FC experiment of U2OS using CL488-82875

    1x10^6 U2OS cells were intracellularly stained with 0.8 ug Coralite® Plus 488 SHPK Recombinant Antibody (CL488-82875, Clone:230115A4)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

☆ 2/28（金）まで！最大30%オフ！ >> トライアルサイズもお得な一次抗体キャンペーン

Tested Applications

Positive FC (Intra) detected inU2OS cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-82875 targets SHPK in FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SHPK fusion protein Ag33893 相同性解析による交差性が予測される生物種
Full Name sedoheptulokinase
Calculated molecular weight 478 aa, 52 kDa
GenBank accession numberBC020543
Gene symbol SHPK
Gene ID (NCBI) 23729
RRIDAB_3673091
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SHPK, also named CARKL, is a carbohydrate kinase involved in the phosphorylation of sugars as they enter a cell, inhibiting return across the cell membrane (PubMed: 10673275). SHPK is a sedoheptulose kinase of the pentose phosphate pathway. It acts as a modulator of macrophage activation through control of glucose metabolism (PMID: 22682222).

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 SHPK antibody CL488-82875Download protocol
Standard Protocols
Click here to view our Standard Protocols