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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using CoraLite® Plus 488-conjugated SIAE Monoclonal anti (CL488-66522)

    IF Staining of PC-3 using CL488-66522

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using CoraLite® Plus 488 SIAE antibody (CL488-66522, Clone: 1G5F7 ) at dilution of 1:600, CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of PC-3 cells using CoraLite® Plus 488-conjugated SIAE Monoclonal anti (CL488-66522)

    FC experiment of PC-3 using CL488-66522

    1X10^6 PC-3 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human SIAE (CL488-66522, Clone:1G5F7) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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CoraLite® Plus 488-conjugated SIAE Monoclonal antibody

SIAE Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-66522
Clone No.1G5F7

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

IF/ICC, FC (Intra)

Synonyms

Sialate O-acetylesterase, LSE, H-Lse, H Lse, EC:3.1.1.53

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inPC-3 cells
Positive FC (Intra) detected inPC-3 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66522 targets SIAE in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SIAE fusion protein Ag14624 相同性解析による交差性が予測される生物種
Full Name sialic acid acetylesterase
Calculated molecular weight 58 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC068450
Gene Symbol SIAE
Gene ID (NCBI) 54414
RRIDAB_3084254
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9HAT2
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 SIAE antibody CL488-66522Download protocol
FC protocol for CL Plus 488 SIAE antibody CL488-66522Download protocol
Standard Protocols
Click here to view our Standard Protocols