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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SIAH1 Recombinant antibody

SIAH1 Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

240377B4

Cat no : 83389-4-RR

Synonyms

Siah-1a, Siah-1, RING-type E3 ubiquitin transferase SIAH1, EC:2.3.2.27, E3 ubiquitin-protein ligase SIAH1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SIAH1 Recombinant antibody (83389-4-RR)

    WB analysis using 83389-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83389-4-RR (SIAH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using SIAH1 Recombinant antibody (83389-4-RR)

    WB analysis of mouse kidney using 83389-4-RR

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 83389-4-RR (SIAH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using SIAH1 Recombinant antibody (83389-4-RR)

    IHC staining of mouse testis using 83389-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83389-4-RR (SIAH1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of A549 cells using SIAH1 Recombinant antibody (83389-4-RR)

    FC experiment of A549 using 83389-4-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug Siah1 Recombinant Antibody (83389-4-RR, Clone:240377B4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83389-4-RR

    Affinity and Kinetic Characterization of 83389-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83389-4-RR against Human SIAH1 were performed. The affinity constant is 2.39 nM.

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Tested Applications

Positive WB detected inA549 cells, mouse kidney tissue, HepG2 cells, HEK-293T cells, SH-SY5Y cells, Jurkat cells, COLO 320 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83389-4-RR is SIAH1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SIAH1 fusion protein Ag34618 相同性解析による交差性が予測される生物種
Full Name seven in absentia homolog 1 (Drosophila)
Calculated molecular weight 282 aa, 31 kDa
Observed molecular weight 30-34 kDa
GenBank accession numberBC035562
Gene symbol SIAH1
Gene ID (NCBI) 6477
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SIAH1, also named as HUMSIAH, Siah-1a and Siah-1S(21kd), belongs to the SINA (Seven in absentia) family. SIAH1 is known to cause indirect degradation of beta-catenin through formation of a complex with Siah-interacting protein (SIP), Skp1 and Ebi. SIAH1 can form heterodimer or homodimer such as Siah-1*Siah-1(70 or 62kd), Siah-1*Siah-1S(42kd) and Siah-1S*Siah-1S(51-56kd). Importantly, results from in vitro soft agar assay demonstrated that Siah-1S displays a promotion effect on cells tumorigenicity. (PMID:17420721)

Protocols

Product Specific Protocols
WB protocol for SIAH1 antibody 83389-4-RRDownload protocol
IHC protocol for SIAH1 antibody 83389-4-RRDownload protocol
FC protocol for SIAH1 antibody 83389-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols