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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SIK1 Recombinant antibody, PBS Only (Capture)

SIK1 Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240961H3

Cat no : 83897-4-PBS

Synonyms

Serine/threonine-protein kinase SIK1, Salt-inducible kinase 1, salt inducible kinase 1, MSK, EC:2.7.11.1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using SIK1 Recombinant antibody (83897-4-RR)

    WB analysis using 83897-4-RR (same clone as 83897-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83897-4-RR (SIK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83897-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using SIK1 Recombinant antibody (83897-4-RR)

    IHC staining of human stomach using 83897-4-RR (same clone as 83897-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 83897-4-RR (SIK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83897-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using SIK1 Recombinant antibody (83897-4-RR)

    IF Staining of A549 using 83897-4-RR (same clone as 83897-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using SIK1 antibody (83897-4-RR, Clone: 240961H3 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83897-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00838-2

    Cytometric bead array standard curve of MP00838-2

    Cytometric bead array standard curve of MP00838-2, SIK1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83897-4-PBS. Detection antibody: 83897-2-PBS. Standard: Ag28481. Range: 0.625-40 ng/mL.

  • Affinity and Kinetic Characterization of 83897-4-RR

    Affinity and Kinetic Characterization of 83897-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83897-4-RR against Human SIK1 were performed. The affinity constant is 0.382 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83897-4-PBS targets SIK1 as part of a matched antibody pair:

MP00838-2: 83897-4-PBS capture and 83897-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SIK1 fusion protein Ag28481 相同性解析による交差性が予測される生物種
Full Name salt-inducible kinase 1
Calculated molecular weight 783 aa, 85 kDa
Observed molecular weight85 kDa
GenBank accession numberBC038504
Gene symbol SIK1
Gene ID (NCBI) 150094
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

SIK1(Salt-inducible kinase 1) is also named SIK, SNF1LK, and belongs to the CAMK Ser/Thr protein kinase family. It associates constitutively with the NK regulatory complex and is responsible for increases in its catalytic activity following small elevations in intracellular sodium concentrations(PMID:17939993). SIK1 is required for the phosphorylation of class II HDACs and expression of MEF2 target genes in myocytes(PMID:17468767). Defects in SIK1 may be associated with some cancers, such as breast cancer. Loss of SIK1 correlates with poor patient outcomes in breast cancers(PMID:19622832).