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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SIK1 Recombinant antibody

SIK1 Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240961H3

Cat no : 83897-4-RR

Synonyms

Serine/threonine-protein kinase SIK1, Salt-inducible kinase 1, salt inducible kinase 1, MSK, EC:2.7.11.1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SIK1 Recombinant antibody (83897-4-RR)

    WB analysis using 83897-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83897-4-RR (SIK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach tissue using SIK1 Recombinant antibody (83897-4-RR)

    IHC staining of human stomach using 83897-4-RR

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 83897-4-RR (SIK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using SIK1 Recombinant antibody (83897-4-RR)

    IF Staining of A549 using 83897-4-RR

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using SIK1 antibody (83897-4-RR, Clone: 240961H3 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 83897-4-RR

    Affinity and Kinetic Characterization of 83897-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83897-4-RR against Human SIK1 were performed. The affinity constant is 0.382 nM.

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Tested Applications

Positive WB detected inHeLa cells, A549 cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83897-4-RR is SIK1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SIK1 fusion protein Ag28481 相同性解析による交差性が予測される生物種
Full Name salt-inducible kinase 1
Calculated molecular weight 783 aa, 85 kDa
Observed molecular weight85 kDa
GenBank accession numberBC038504
Gene symbol SIK1
Gene ID (NCBI) 150094
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SIK1(Salt-inducible kinase 1) is also named SIK, SNF1LK, and belongs to the CAMK Ser/Thr protein kinase family. It associates constitutively with the NK regulatory complex and is responsible for increases in its catalytic activity following small elevations in intracellular sodium concentrations(PMID:17939993). SIK1 is required for the phosphorylation of class II HDACs and expression of MEF2 target genes in myocytes(PMID:17468767). Defects in SIK1 may be associated with some cancers, such as breast cancer. Loss of SIK1 correlates with poor patient outcomes in breast cancers(PMID:19622832).

Protocols

Product Specific Protocols
WB protocol for SIK1 antibody 83897-4-RRDownload protocol
IHC protocol for SIK1 antibody 83897-4-RRDownload protocol
IF protocol for SIK1 antibody 83897-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols