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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated SIN3A Monoclonal antibody

SIN3A Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2B12E8

Cat no : CL488-67197

Synonyms

DKFZp434K2235, FLJ90319, KIAA0700, SIN3A

Validation Data Gallery

Applications:
  • FC experiment of A431 using CL488-67197

    FC experiment of A431 using CL488-67197

    1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human SIN3A (CL488-67197, Clone:2B12E8) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive FC detected inA431 cells
For other applications, we recommend the unconjugated version of this antibody, 67197-1-Ig

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-67197 targets SIN3A in FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SIN3A fusion protein Ag28865 相同性解析による交差性が予測される生物種
Full Name SIN3 homolog A, transcription regulator (yeast)
Calculated molecular weight 145 kDa
Observed molecular weight 145-150 kDa
GenBank accession numberBC066364
Gene symbol SIN3A
Gene ID (NCBI) 25942
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 SIN3A antibody CL488-67197Download protocol
Standard Protocols
Click here to view our Standard Protocols