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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SIRP Alpha/CD172a Monoclonal antibody (67337-1-Ig)

    WB analysis using 67337-1-Ig (same clone as 67337-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67337-1-Ig (SIRP Alpha/CD172a antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67337-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SW480 cells using SIRP Alpha/CD172a Monoclonal antibody (67337-1-Ig)

    WB analysis of SW480 using 67337-1-Ig (same clone as 67337-1-PBS)

    SW480 cells were subjected to SDS PAGE followed by western blot with 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67337-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HL-60 cells using SIRP Alpha/CD172a Monoclonal antibody (67337-1-Ig)

    WB analysis of HL-60 using 67337-1-Ig (same clone as 67337-1-PBS)

    HL-60 cells were subjected to SDS PAGE followed by western blot with 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67337-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U-251 cells using SIRP Alpha/CD172a Monoclonal antibody (67337-1-Ig)

    WB analysis of U-251 using 67337-1-Ig (same clone as 67337-1-PBS)

    U-251 cells were subjected to SDS PAGE followed by western blot with 67337-1-Ig (SIRP alpha antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67337-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of THP-1 cells using SIRP Alpha/CD172a Monoclonal antibody (67337-1-Ig)

    WB analysis of THP-1 using 67337-1-Ig (same clone as 67337-1-PBS)

    THP-1 cells were subjected to SDS PAGE followed by western blot with 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67337-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat cerebellum tissue using SIRP Alpha/CD172a Monoclonal antibody (67337-1-Ig)

    IHC staining of rat cerebellum using 67337-1-Ig (same clone as 67337-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67337-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat cerebellum tissue using SIRP Alpha/CD172a Monoclonal antibody (67337-1-Ig)

    IHC staining of rat cerebellum using 67337-1-Ig (same clone as 67337-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67337-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using SIRP Alpha/CD172a Monoclonal antibody (67337-1-Ig)

    IHC staining of rat brain using 67337-1-Ig (same clone as 67337-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67337-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using SIRP Alpha/CD172a Monoclonal antibody (67337-1-Ig)

    IHC staining of rat brain using 67337-1-Ig (same clone as 67337-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67337-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using SIRP Alpha/CD172a Monoclonal antibody (67337-1-Ig)

    IHC staining of human liver using 67337-1-Ig (same clone as 67337-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67337-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using SIRP Alpha/CD172a Monoclonal antibody (67337-1-Ig)

    IHC staining of human liver using 67337-1-Ig (same clone as 67337-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67337-1-PBS in a different storage buffer formulation.

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SIRP Alpha/CD172a Monoclonal antibody, PBS Only

SIRP Alpha/CD172a Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 67337-1-PBS
Clone No.2F8H3

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, Indirect ELISA

Synonyms

SIRP Alpha, SIRPA, Inhibitory receptor SHPS-1, CD172a, CD172 antigen-like family member A

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67337-1-PBS targets SIRP Alpha/CD172a in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen SIRP Alpha/CD172a fusion protein Ag6361 相同性解析による交差性が予測される生物種
Full Name signal-regulatory protein alpha
Calculated molecular weight 55 kDa
Observed molecular weight 75-85 kDa
GenBank accession numberBC033092
Gene Symbol SIRP Alpha
Gene ID (NCBI) 140885
RRIDAB_2882595
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP78324
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

SIRP Alpha, also known as CD172a, SHPS-1, and BIT, belongs to the SIRP family. SIRP Alpha is a transmembrane glycoprotein expressed explicitly on myeloid cells and provides a "do not eat me" signal after engaging with integrin-associated protein CD47 on tumor cells (PMID: 36419386). SIRP Alpha shows heterogeneity in molecular weight (~65-120 kDa) in various tissues due to differential glycosylation (PMID: 18051954). This antibody detects a 75-85 kDa band.