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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SIRP Alpha Monoclonal antibody, PBS Only

SIRP Alpha Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat, Pig, Rabbit

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F8H3

Cat no : 67337-1-PBS

Synonyms

BIT, CD172A, Inhibitory receptor SHPS 1, Macrophage fusion receptor, MFR, MYD 1, MyD 1 antigen, MYD1, P84, PTPNS1, SHP substrate 1, SHPS 1, SHPS1, SIRP, SIRP Alpha, SIRP ALPHA 1, Sirp alpha 2, Sirp alpha 3, SIRPA, SIRPalpha, SIRPalpha2

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using SIRP Alpha Monoclonal antibody (67337-1-Ig)

    WB analysis using 67337-1-Ig (same clone as 67337-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67337-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SW480 cells using SIRP Alpha Monoclonal antibody (67337-1-Ig)

    WB analysis of SW480 using 67337-1-Ig (same clone as 67337-1-PBS)

    SW480 cells were subjected to SDS PAGE followed by western blot with 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67337-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HL-60 cells using SIRP Alpha Monoclonal antibody (67337-1-Ig)

    WB analysis of HL-60 using 67337-1-Ig (same clone as 67337-1-PBS)

    HL-60 cells were subjected to SDS PAGE followed by western blot with 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67337-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U-251 cells using SIRP Alpha Monoclonal antibody (67337-1-Ig)

    WB analysis of U-251 using 67337-1-Ig (same clone as 67337-1-PBS)

    U-251 cells were subjected to SDS PAGE followed by western blot with 67337-1-Ig (SIRP alpha antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67337-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of THP-1 cells using SIRP Alpha Monoclonal antibody (67337-1-Ig)

    WB analysis of THP-1 using 67337-1-Ig (same clone as 67337-1-PBS)

    THP-1 cells were subjected to SDS PAGE followed by western blot with 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67337-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67337-1-PBS targets SIRP Alpha in WB, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig, Rabbit samples.

Tested Reactivity Human, Mouse, Rat, Pig, Rabbit
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen SIRP Alpha fusion protein Ag6361 相同性解析による交差性が予測される生物種
Full Name signal-regulatory protein alpha
Calculated molecular weight 55 kDa
Observed molecular weight 75-85 kDa
GenBank accession numberBC033092
Gene symbol SIRPA
Gene ID (NCBI) 140885
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

SIRP Alpha, also known as CD172a, SHPS-1, and BIT, belongs to the SIRP family. SIRP Alpha is a transmembrane glycoprotein expressed explicitly on myeloid cells and provides a "do not eat me" signal after engaging with integrin-associated protein CD47 on tumor cells (PMID: 36419386). SIRP Alpha shows heterogeneity in molecular weight (~65-120 kDa) in various tissues due to differential glycosylation (PMID: 18051954). This antibody detects a 75-85 kDa band.