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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SIRP Alpha/CD172a Monoclonal antibody

SIRP Alpha/CD172a Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2F8H3

Cat no : 67337-1-Ig

Synonyms

SIRP Alpha, SIRPA, Inhibitory receptor SHPS-1, CD172a, CD172 antigen-like family member A

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SIRP Alpha/CD172a Monoclonal antibody (67337-1-Ig)

    WB analysis using 67337-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67337-1-Ig (SIRP Alpha/CD172a antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SW480 cells using SIRP Alpha/CD172a Monoclonal antibody (67337-1-Ig)

    WB analysis of SW480 using 67337-1-Ig

    SW480 cells were subjected to SDS PAGE followed by western blot with 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using SIRP Alpha/CD172a Monoclonal antibody (67337-1-Ig)

    WB analysis of HL-60 using 67337-1-Ig

    HL-60 cells were subjected to SDS PAGE followed by western blot with 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of THP-1 cells using SIRP Alpha/CD172a Monoclonal antibody (67337-1-Ig)

    WB analysis of THP-1 using 67337-1-Ig

    THP-1 cells were subjected to SDS PAGE followed by western blot with 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U-251 cells using SIRP Alpha/CD172a Monoclonal antibody (67337-1-Ig)

    WB analysis of U-251 using 67337-1-Ig

    U-251 cells were subjected to SDS PAGE followed by western blot with 67337-1-Ig (SIRP alpha antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat cerebellum tissue using SIRP Alpha/CD172a Monoclonal antibody (67337-1-Ig)

    IHC staining of rat cerebellum using 67337-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat cerebellum tissue using SIRP Alpha/CD172a Monoclonal antibody (67337-1-Ig)

    IHC staining of rat cerebellum using 67337-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using SIRP Alpha/CD172a Monoclonal antibody (67337-1-Ig)

    IHC staining of human liver using 67337-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using SIRP Alpha/CD172a Monoclonal antibody (67337-1-Ig)

    IHC staining of human liver using 67337-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat brain tissue using SIRP Alpha/CD172a Monoclonal antibody (67337-1-Ig)

    IHC staining of rat brain using 67337-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat brain tissue using SIRP Alpha/CD172a Monoclonal antibody (67337-1-Ig)

    IHC staining of rat brain using 67337-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inpig brain tissue, U-251 cells, SW480 cells, HL-60 cells, THP-1 cells, rat brain tissue, mouse brain tissue, rabbit brain tissue
Positive IHC detected inrat cerebellum tissue, human liver tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67337-1-Ig targets SIRP Alpha/CD172a in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen SIRP Alpha/CD172a fusion protein Ag6361 相同性解析による交差性が予測される生物種
Full Name signal-regulatory protein alpha
Calculated molecular weight 55 kDa
Observed molecular weight 75-85 kDa
GenBank accession numberBC033092
Gene symbol SIRP Alpha
Gene ID (NCBI) 140885
RRIDAB_2882595
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SIRP Alpha, also known as CD172a, SHPS-1, and BIT, belongs to the SIRP family. SIRP Alpha is a transmembrane glycoprotein expressed explicitly on myeloid cells and provides a "do not eat me" signal after engaging with integrin-associated protein CD47 on tumor cells (PMID: 36419386). SIRP Alpha shows heterogeneity in molecular weight (~65-120 kDa) in various tissues due to differential glycosylation (PMID: 18051954). This antibody detects a 75-85 kDa band.

Protocols

Product Specific Protocols
WB protocol for SIRP Alpha/CD172a antibody 67337-1-IgDownload protocol
IHC protocol for SIRP Alpha/CD172a antibody 67337-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols