Validation Data Gallery
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Tested Applications
| Positive WB detected in | rat cerebellum tissue, rabbit cerebellum tissue, pig cerebellum tissue, mouse brain tissue, rat brain tissue, rabbit brain tissue, pig brain tissue, U-937 cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | OS-RC-2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68206-1-Ig targets SIRP beta 1/CD172b in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.
| Tested Reactivity | human, mouse, rat, pig, rabbit |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | SIRP beta 1/CD172b fusion protein Ag28615 相同性解析による交差性が予測される生物種 |
| Full Name | signal-regulatory protein beta 1 |
| Calculated molecular weight | 43 kDa |
| Observed molecular weight | 43 kDa |
| GenBank accession number | BC025286 |
| Gene Symbol | SIRPB1 |
| Gene ID (NCBI) | 10326 |
| RRID | AB_2935294 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | O00241 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SIRP Beta 1 is a member of the signal-regulatory-protein (SIRP) family and also belongs to the immunoglobulin superfamily. SIRP family members are cell surface signaling receptors differentially expressed in leukocytes and the central nervous system. SIRP Beta 1 interacts with TYROBP/DAP12, a protein-bearing immunoreceptor tyrosine-based activation motif. SIRP Beta 1 also participates in the recruitment of tyrosine kinase SYK.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SIRP beta 1/CD172b antibody 68206-1-Ig | Download protocol |
| IHC protocol for SIRP beta 1/CD172b antibody 68206-1-Ig | Download protocol |
| IF protocol for SIRP beta 1/CD172b antibody 68206-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |