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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SIRP beta 1/CD172b Monoclonal antibody

SIRP beta 1/CD172b Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3A3H1

Cat no : 68206-1-Ig

Synonyms

SIRPB1, SIRP Beta 1, SIRP-beta-1, Signal-regulatory protein beta-1, CD172b

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SIRP beta 1/CD172b Monoclonal antibody (68206-1-Ig)

    WB analysis using 68206-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68206-1-Ig (SIRP Beta 1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using SIRP beta 1/CD172b Monoclonal antibody (68206-1-Ig)

    IHC staining of human lung cancer using 68206-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68206-1-Ig (SIRP Beta 1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of OS-RC-2 cells using SIRP beta 1/CD172b Monoclonal antibody (68206-1-Ig)

    IF Staining of OS-RC-2 using 68206-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed OS-RC-2 cells using SIRP Beta 1 antibody (68206-1-Ig, Clone: 3A3H1 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

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Tested Applications

Positive WB detected inrat cerebellum tissue, rabbit cerebellum tissue, pig cerebellum tissue, mouse brain tissue, rat brain tissue, rabbit brain tissue, pig brain tissue, U-937 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inOS-RC-2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68206-1-Ig targets SIRP beta 1/CD172b in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SIRP beta 1/CD172b fusion protein Ag28615 相同性解析による交差性が予測される生物種
Full Name signal-regulatory protein beta 1
Calculated molecular weight 43 kDa
Observed molecular weight 43 kDa
GenBank accession numberBC025286
Gene symbol SIRPB1
Gene ID (NCBI) 10326
RRIDAB_2935294
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SIRP Beta 1 is a member of the signal-regulatory-protein (SIRP) family and also belongs to the immunoglobulin superfamily. SIRP family members are cell surface signaling receptors differentially expressed in leukocytes and the central nervous system. SIRP Beta 1 interacts with TYROBP/DAP12, a protein-bearing immunoreceptor tyrosine-based activation motif. SIRP Beta 1 also participates in the recruitment of tyrosine kinase SYK.

Protocols

Product Specific Protocols
WB protocol for SIRP beta 1/CD172b antibody 68206-1-IgDownload protocol
IHC protocol for SIRP beta 1/CD172b antibody 68206-1-IgDownload protocol
IF protocol for SIRP beta 1/CD172b antibody 68206-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols