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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SIRP beta 1/CD172b Monoclonal antibody (68206-1-Ig)

    WB analysis using 68206-1-Ig (same clone as 68206-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68206-1-Ig (SIRP Beta 1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68206-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using SIRP beta 1/CD172b Monoclonal antibody (68206-1-Ig)

    IHC staining of human lung cancer using 68206-1-Ig (same clone as 68206-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68206-1-Ig (SIRP Beta 1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68206-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of OS-RC-2 cells using SIRP beta 1/CD172b Monoclonal antibody (68206-1-Ig)

    IF Staining of OS-RC-2 using 68206-1-Ig (same clone as 68206-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed OS-RC-2 cells using SIRP Beta 1 antibody (68206-1-Ig, Clone: 3A3H1 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 68206-1-PBS in a different storage buffer formulation.

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SIRP beta 1/CD172b Monoclonal antibody, PBS Only

SIRP beta 1/CD172b Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 68206-1-PBS
Clone No.3A3H1

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

SIRPB1, SIRP Beta 1, SIRP-beta-1, Signal-regulatory protein beta-1, CD172b

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68206-1-PBS targets SIRP beta 1/CD172b in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SIRP beta 1/CD172b fusion protein Ag28615 相同性解析による交差性が予測される生物種
Full Name signal-regulatory protein beta 1
Calculated molecular weight 43 kDa
Observed molecular weight 43 kDa
GenBank accession numberBC025286
Gene Symbol SIRPB1
Gene ID (NCBI) 10326
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO00241
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

SIRP Beta 1 is a member of the signal-regulatory-protein (SIRP) family and also belongs to the immunoglobulin superfamily. SIRP family members are cell surface signaling receptors differentially expressed in leukocytes and the central nervous system. SIRP Beta 1 interacts with TYROBP/DAP12, a protein-bearing immunoreceptor tyrosine-based activation motif. SIRP Beta 1 also participates in the recruitment of tyrosine kinase SYK.