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CoraLite® Plus 750-conjugated SIRT1 Polyclonal antibody
SIRT1 Polyclonal Antibody for WB, FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, FC (Intra)
Conjugate
CoraLite® Plus 750 Fluorescent Dye
Cat no : CL750-13161
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, MDA-MB-231 cells |
Positive FC (Intra) detected in | A431 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL750-13161 targets SIRT1 in WB, FC (Intra) applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | SIRT1 fusion protein Ag3808 相同性解析による交差性が予測される生物種 |
Full Name | sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae) |
Calculated molecular weight | 747 aa, 82 kDa |
Observed molecular weight | 110-130 kDa, 80-85 kDa |
GenBank accession number | BC012499 |
Gene symbol | SIRT1 |
Gene ID (NCBI) | 23411 |
Conjugate | CoraLite® Plus 750 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 755 nm / 780 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SIRT1, also named as SIR2L1, contains a deacetylase sirtuin-type domain and belongs to the sirtuin family. The post-translation modified SIRT1 is a 110-130 kDa protein, which contains one deacetylase sirtuin-type domain. The 75-80 kDa SirT1 fragment was detected to lack the carboxy-terminus (PMID:21305533). SirT1 exists a 57-61 kDa isoform. SIRT1 may be found in nucleolus, nuclear euchromatin, heterochromatin, and inner membrane. It can shuttle between the nucleus and cytoplasm. SIRT1 regulates processes such as apoptosis and muscle differentiation by deacetylating key proteins. SIRT1 in particular initiates several signaling events relevant to cardioprotection, including activation of endothelial nitric oxide synthase, INS receptor signaling, and autophagy. In addition, SIRT1 activation elicits resistance to oxidative stress via the regulation of transcription factors and co-activators such as FOXO, Hif-2a, and NF-kB. SIRT1 regulates the p53-dependent DNA damage response pathway by binding to and deacetylating p53, specifically at Lysine 382. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human SIRT1.
Protocols
Product Specific Protocols | |
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WB protocol for CL Plus 750 SIRT1 antibody CL750-13161 | Download protocol |
FC protocol for CL Plus 750 SIRT1 antibody CL750-13161 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |