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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SIRT2 Monoclonal antibody (66410-1-Ig)

    WB analysis using 66410-1-Ig (same clone as 66410-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66410-1-Ig (SIRT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66410-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using SIRT2 Monoclonal antibody (66410-1-Ig)

    WB analysis of MCF-7 using 66410-1-Ig (same clone as 66410-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66410-1-Ig (SIRT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66410-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using SIRT2 Monoclonal antibody (66410-1-Ig)

    WB analysis of mouse brain using 66410-1-Ig (same clone as 66410-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 66410-1-Ig (SIRT2 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66410-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using SIRT2 Monoclonal antibody (66410-1-Ig)

    WB analysis of rat brain using 66410-1-Ig (same clone as 66410-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 66410-1-Ig (SIRT2 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66410-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat heart tissue using SIRT2 Monoclonal antibody (66410-1-Ig)

    WB analysis of rat heart using 66410-1-Ig (same clone as 66410-1-PBS)

    rat heart tissue were subjected to SDS PAGE followed by western blot with 66410-1-Ig (SIRT2 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66410-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig brain tissue using SIRT2 Monoclonal antibody (66410-1-Ig)

    WB analysis of pig brain using 66410-1-Ig (same clone as 66410-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 66410-1-Ig (SIRT2 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66410-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig heart tissue using SIRT2 Monoclonal antibody (66410-1-Ig)

    WB analysis of pig heart using 66410-1-Ig (same clone as 66410-1-PBS)

    pig heart tissue were subjected to SDS PAGE followed by western blot with 66410-1-Ig (SIRT2 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66410-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of fetal human brain tissue using SIRT2 Monoclonal antibody (66410-1-Ig)

    WB analysis of fetal human brain using 66410-1-Ig (same clone as 66410-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66410-1-Ig (SIRT2 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66410-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using SIRT2 Monoclonal antibody (66410-1-Ig)

    WB analysis of rat brain using 66410-1-Ig (same clone as 66410-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 66410-1-Ig (SIRT2 Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66410-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using SIRT2 Monoclonal antibody (66410-1-Ig)

    WB analysis of mouse brain using 66410-1-Ig (same clone as 66410-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 66410-1-Ig (SIRT2 Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66410-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using SIRT2 Monoclonal antibody (66410-1-Ig)

    IHC staining of human kidney using 66410-1-Ig (same clone as 66410-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66410-1-Ig (SIRT2 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66410-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using SIRT2 Monoclonal antibody (66410-1-Ig)

    IHC staining of human heart using 66410-1-Ig (same clone as 66410-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66410-1-Ig (SIRT2 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66410-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using SIRT2 Monoclonal antibody (66410-1-Ig)

    IHC staining of human heart using 66410-1-Ig (same clone as 66410-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66410-1-Ig (SIRT2 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66410-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using SIRT2 Monoclonal antibody (66410-1-Ig)

    IHC staining of human kidney using 66410-1-Ig (same clone as 66410-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66410-1-Ig (SIRT2 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66410-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SIRT2 Monoclonal antibody (66410-1-Ig)

    IF Staining of HepG2 using 66410-1-Ig (same clone as 66410-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed HepG2 cells using 66410-1-Ig(SIRT2 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66410-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

SIRT2 Monoclonal antibody, PBS Only

SIRT2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66410-1-PBS
Clone No.1D8G10

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

SIR2, SIR2 like protein 2, SIR2L, SIR2L2, SIRT2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66410-1-PBS targets SIRT2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SIRT2 fusion protein Ag7756 相同性解析による交差性が予測される生物種
Full Name sirtuin (silent mating type information regulation 2 homolog) 2 (S. cerevisiae)
Calculated molecular weight 43 kDa
Observed molecular weight 37-45 kDa
GenBank accession numberBC003547
Gene Symbol SIRT2
Gene ID (NCBI) 22933
RRIDAB_2881782
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8IXJ6
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

The silent information regulator(SIR2) family of genes is highly conserved from prokaryotes to eukaryotes and is involved in diverse processes, including transcriptional regulation, cell cycle progression, DNA damage repair, and aging. SIR2 contains a 323 amino acid catalytic core domain with a NAD-binding domain and a large groove which is the likely site of catalysis. SIR2 is widely expressed and highly expressed in the heart, brain, and skeletal muscle, while it is weakly expressed in the placenta and lungs. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of the microtubule network. SIRT2 exists in various isoforms and the molecular weight of isoforms are 35 kDa, 40 kDa, and 42 kDa.