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KD/KO Validated

SIRT2 Monoclonal antibody

Name: SIRT2 Monoclonal antibody
Brand: Proteintech
SKU: 66410-1-Ig

SIRT2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

CloneNo.

1D8G10

Review Product

Cat no : 66410-1-Ig

Synonyms

SIR2, SIR2 like protein 2, SIR2L, SIR2L2, SIRT2

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis using 66410-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 66410-1-Ig (SIRT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.
WB analysis of MCF-7 using 66410-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 66410-1-Ig (SIRT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of pig heart using 66410-1-Ig
pig heart tissue were subjected to SDS PAGE followed by western blot with 66410-1-Ig (SIRT2 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of fetal human brain using 66410-1-Ig
fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66410-1-Ig (SIRT2 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 66410-1-Ig
rat brain tissue were subjected to SDS PAGE followed by western blot with 66410-1-Ig (SIRT2 Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 66410-1-Ig
mouse brain tissue were subjected to SDS PAGE followed by western blot with 66410-1-Ig (SIRT2 Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 66410-1-Ig
mouse brain tissue were subjected to SDS PAGE followed by western blot with 66410-1-Ig (SIRT2 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 66410-1-Ig
rat brain tissue were subjected to SDS PAGE followed by western blot with 66410-1-Ig (SIRT2 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of rat heart using 66410-1-Ig
rat heart tissue were subjected to SDS PAGE followed by western blot with 66410-1-Ig (SIRT2 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of pig brain using 66410-1-Ig
pig brain tissue were subjected to SDS PAGE followed by western blot with 66410-1-Ig (SIRT2 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IHC staining of human kidney using 66410-1-Ig
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66410-1-Ig (SIRT2 Antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human heart using 66410-1-Ig
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66410-1-Ig (SIRT2 Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human heart using 66410-1-Ig
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66410-1-Ig (SIRT2 Antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human kidney using 66410-1-Ig
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66410-1-Ig (SIRT2 Antibody) at dilution of 1:200 (under 10x lens).
IF Staining of HepG2 using 66410-1-Ig
Immunofluorescent analysis of (-20℃ Ethanol ) fixed HepG2 cells using 66410-1-Ig(SIRT2 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).

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Cat No. 66410-1-Ig

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	LNCaP cells, fetal human brain tissue, pig heart tissue, pig brain tissue, rat heart tissue, rat brain tissue, mouse brain tissue, MCF-7 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells
Positive IHC detected in	human kidney tissue, human heart tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 6 publications below
IHC	See 4 publications below
IF	See 2 publications below

Product Information

66410-1-Ig targets SIRT2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity	human, mouse, rat, pig
Cited Reactivity	human, mouse, rat
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	SIRT2 fusion protein Ag7756 相同性解析による交差性が予測される生物種
Full Name	sirtuin (silent mating type information regulation 2 homolog) 2 (S. cerevisiae)
Calculated molecular weight	43 kDa
Observed molecular weight	37-45 kDa
GenBank accession number	BC003547
Gene symbol	SIRT2
Gene ID (NCBI)	22933
RRID	AB_2881782
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The silent information regulator(SIR2) family of genes is highly conserved from prokaryotes to eukaryotes and is involved in diverse processes, including transcriptional regulation, cell cycle progression, DNA damage repair, and aging. SIR2 contains a 323 amino acid catalytic core domain with a NAD-binding domain and a large groove which is the likely site of catalysis. SIR2 is widely expressed and highly expressed in the heart, brain, and skeletal muscle, while it is weakly expressed in the placenta and lungs. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of the microtubule network. SIRT2 exists in various isoforms and the molecular weight of isoforms are 35 kDa, 40 kDa, and 42 kDa.

Protocols

Product Specific Protocols
WB protocol for SIRT2 antibody 66410-1-Ig	Download protocol
IHC protocol for SIRT2 antibody 66410-1-Ig	Download protocol
IF protocol for SIRT2 antibody 66410-1-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Redox Biol A redox-mediated conformational change in NQO1 controls binding to microtubules and α-tubulin acetylation. Authors - David Siegel KD Validated View Article
rat	WB,IHC	CNS Neurosci Ther Proteomic analysis of the effects of caffeine in a neonatal rat model of hypoxic-ischemic white matter damage. Authors - Liu Yang View Article
human	WB	Prostate SIRT2 promotes cell proliferation and migration through mediating ERK1/2 activation and lactosylceramide accumulation in prostate cancer Authors - Rui Lin KD Validated View Article
human	WB	PLoS Pathog Bacterial infection promotes tumorigenesis of colorectal cancer via regulating CDC42 acetylation Authors - Dan-Ni Wang KD Validated View Article
human	IF	Oncotarget Development of a multiplex assay to assess activated p300/CBP in circulating prostate tumor cells Authors - Mikolaj Filon View Article
human,mouse	WB	Cell Death Dis FBXO31 is upregulated by METTL3 to promote pancreatic cancer progression via regulating SIRT2 ubiquitination and degradation Authors - Kai Chen View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SIRT2 Monoclonal antibody

SIRT2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis using 66410-1-Ig

WB analysis of MCF-7 using 66410-1-Ig

WB analysis of pig heart using 66410-1-Ig

WB analysis of fetal human brain using 66410-1-Ig

WB analysis of rat brain using 66410-1-Ig

WB analysis of mouse brain using 66410-1-Ig

WB analysis of mouse brain using 66410-1-Ig

WB analysis of rat brain using 66410-1-Ig

WB analysis of rat heart using 66410-1-Ig

WB analysis of pig brain using 66410-1-Ig

IHC staining of human kidney using 66410-1-Ig

IHC staining of human heart using 66410-1-Ig

IHC staining of human heart using 66410-1-Ig

IHC staining of human kidney using 66410-1-Ig

IF Staining of HepG2 using 66410-1-Ig

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

A redox-mediated conformational change in NQO1 controls binding to microtubules and α-tubulin acetylation.

Proteomic analysis of the effects of caffeine in a neonatal rat model of hypoxic-ischemic white matter damage.

SIRT2 promotes cell proliferation and migration through mediating ERK1/2 activation and lactosylceramide accumulation in prostate cancer

Bacterial infection promotes tumorigenesis of colorectal cancer via regulating CDC42 acetylation

Development of a multiplex assay to assess activated p300/CBP in circulating prostate tumor cells

FBXO31 is upregulated by METTL3 to promote pancreatic cancer progression via regulating SIRT2 ubiquitination and degradation