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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated SIRT2 Monoclonal ant (CL488-66410)

    IF Staining of HepG2 using CL488-66410

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL488-66410 (SIRT2 antibody) at dilution of 1:100.

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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated SIRT2 Monoclonal antibody

SIRT2 Monoclonal Antibody for IF/ICC
Cat No. CL488-66410
Clone No.1D8G10

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

IF/ICC

Synonyms

SIR2, SIR2 like protein 2, SIR2L, SIR2L2, SIRT2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66410 targets SIRT2 in IF/ICC applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SIRT2 fusion protein Ag7756 相同性解析による交差性が予測される生物種
Full Name sirtuin (silent mating type information regulation 2 homolog) 2 (S. cerevisiae)
Calculated molecular weight 43 kDa
Observed molecular weight 37-45 kDa
GenBank accession numberBC003547
Gene Symbol SIRT2
Gene ID (NCBI) 22933
RRIDAB_2883329
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8IXJ6
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The silent information regulator(SIR2) family of genes are highly conserved from prokaryotes to eukaryotes and are involved in diverse processes, including transcriptional regulation, cell cycle progression, DNA-damage repair and aging. SIR2 contains a 323 amino acid catalytic core domain with a NAD-binding domain and a large groove which is the likely site of catalysis. SIR2 is widely expressed, highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network. SIRT2 exists various isoforms and the molecular weight of isoforms are 35kDa, 40 kDa, and 42 kDa.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 SIRT2 antibody CL488-66410Download protocol
Standard Protocols
Click here to view our Standard Protocols