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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SIRT5 Recombinant antibody (82246-1-RR)

    WB analysis using 82246-1-RR (same clone as 82246-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82246-1-RR (SIRT5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82246-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SIRT5 Recombinant antibody (82246-1-RR)

    IF Staining of HeLa using 82246-1-RR (same clone as 82246-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SIRT5 antibody (82246-1-RR, Clone: 7L20 ) at dilution of 1:1200 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004). This data was developed using the same antibody clone with 82246-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82246-1-PBS

    Affinity and Kinetic Characterization of 82246-1-PBS

    Biolayer interferometry (BLl) kinetic assays of 82246-1-RR against Human SIRT5 were performed. The affinity constant is 1.64 nM.

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SIRT5 Recombinant antibody, PBS Only

SIRT5 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 82246-1-PBS
Clone No.7L20

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

FLJ36950, SIR2 like protein 5, SIR2L5, SIRT5

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82246-1-PBS targets SIRT5 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SIRT5 fusion protein Ag7195 相同性解析による交差性が予測される生物種
Full Name sirtuin (silent mating type information regulation 2 homolog) 5 (S. cerevisiae)
Calculated molecular weight 34 kDa
Observed molecular weight 32-33 kDa
GenBank accession numberBC000126
Gene Symbol SIRT5
Gene ID (NCBI) 23408
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NXA8
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

SIRT5, a member fo sirtuin family that is a mitochondrial matrix NAD(+)-dependent deacetylase and mono-ADP-ribosyltransferases. Sirtuins play important role in various cellular processed, such as aging, gene silencing and metabolism. SIRT5 mainly locates in cytoplasm. It is an efficient protein lysine desuccinylase and demalonylase. During prolonged fasting, it can activate CPS1, the committed and regulated enzyme of the urea cycle, and this contributes to the regulation of blood ammonia levels.