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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SIRT5 Recombinant antibody

SIRT5 Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

7L20

Cat no : 82246-1-RR

Synonyms

FLJ36950, SIR2 like protein 5, SIR2L5, SIRT5

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SIRT5 Recombinant antibody (82246-1-RR)

    WB analysis using 82246-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82246-1-RR (SIRT5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SIRT5 Recombinant antibody (82246-1-RR)

    IF Staining of HeLa using 82246-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SIRT5 antibody (82246-1-RR, Clone: 7L20 ) at dilution of 1:1200 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004).

  • Affinity and Kinetic Characterization of 82246-1-RR

    Affinity and Kinetic Characterization of 82246-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82246-1-RR against Human SIRT5 were performed. The affinity constant is 1.64 nM.

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Tested Applications

Positive WB detected inA549 cells, HepG2 cells, Jurkat cells, HCT 116 cells, RAW 264.7 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:600-1:2400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82246-1-RR targets SIRT5 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SIRT5 fusion protein Ag7195 相同性解析による交差性が予測される生物種
Full Name sirtuin (silent mating type information regulation 2 homolog) 5 (S. cerevisiae)
Calculated molecular weight 34 kDa
Observed molecular weight 32-33 kDa
GenBank accession numberBC000126
Gene symbol SIRT5
Gene ID (NCBI) 23408
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SIRT5, a member fo sirtuin family that is a mitochondrial matrix NAD(+)-dependent deacetylase and mono-ADP-ribosyltransferases. Sirtuins play important role in various cellular processed, such as aging, gene silencing and metabolism. SIRT5 mainly locates in cytoplasm. It is an efficient protein lysine desuccinylase and demalonylase. During prolonged fasting, it can activate CPS1, the committed and regulated enzyme of the urea cycle, and this contributes to the regulation of blood ammonia levels.

Protocols

Product Specific Protocols
WB protocol for SIRT5 antibody 82246-1-RRDownload protocol
IF protocol for SIRT5 antibody 82246-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols