CoraLite®594-conjugated SIX2 Monoclonal antibody
SIX2 Monoclonal Antibody for FC (Intra)
Host / Isotype
Mouse / IgG2b
Reactivity
human, rat, Canine, mouse, pig
Applications
FC (Intra)
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
1F9G11
Cat no : CL594-66347
Synonyms
Validation Data Gallery
Tested Applications
| Positive FC (Intra) detected in | C2C12 cells |
| Positive FC detected in | C2C12 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| Flow Cytometry (FC) | FC : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL594-66347 targets SIX2 in FC (Intra) applications and shows reactivity with human, rat, Canine, mouse, pig samples.
| Tested Reactivity | human, rat, Canine, mouse, pig |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | SIX2 fusion protein Ag2124 相同性解析による交差性が予測される生物種 |
| Full Name | SIX homeobox 2 |
| Calculated molecular weight | 291 aa, 32 kDa |
| Observed molecular weight | 37 kDa |
| GenBank accession number | BC024033 |
| Gene symbol | SIX2 |
| Gene ID (NCBI) | 10736 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The SIX proteins (sine oculis) are a family of homeodomain transcription factors that share a conserved DNA binding domain. Six members (Six1-Six6) of the Six gene family have been identified in mice and humans. SIX2, containing one homeobox DNA-binding domain, is highly expressed in fetal tissues but expression is limited in adult tissues. SIX2 may be involved in limb tendon and ligament development[PMID:21420949]. It has been previously shown that SIX2 is expressed in developing mesenchymal tissue including head and urogenital system at the time of overt midfacial and renal differentiation[PMID: 22282599].
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL594 SIX2 antibody CL594-66347 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
