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Validation Data Gallery

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  • Flow cytometry (FC) experiment of C2C12 cells using CoraLite®594-conjugated SIX2 Monoclonal antibody (CL594-66347)

    FC experiment of C2C12 using CL594-66347

    1X10^6 C2C12 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human SIX2 (CL594-66347, Clone:1F9G11) (red), or 0.8 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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CoraLite®594-conjugated SIX2 Monoclonal antibody

SIX2 Monoclonal Antibody for FC (Intra)
Cat No. CL594-66347
Clone No.1F9G11

Host / Isotype

Mouse / IgG2b

Reactivity

human, rat, Canine, mouse, pig

Applications

FC (Intra)

Synonyms

Homeobox protein SIX2, Sine oculis homeobox homolog 2, SIX homeobox 2, SIX2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive FC (Intra) detected inC2C12 cells
Positive FC detected inC2C12 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66347 targets SIX2 in FC (Intra) applications and shows reactivity with human, rat, Canine, mouse, pig samples.

Tested Reactivity human, rat, Canine, mouse, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen SIX2 fusion protein Ag2124 相同性解析による交差性が予測される生物種
Full Name SIX homeobox 2
Calculated molecular weight 291 aa, 32 kDa
Observed molecular weight 37 kDa
GenBank accession numberBC024033
Gene Symbol SIX2
Gene ID (NCBI) 10736
RRIDAB_3084706
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NPC8
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The SIX proteins (sine oculis) are a family of homeodomain transcription factors that share a conserved DNA binding domain. Six members (Six1-Six6) of the Six gene family have been identified in mice and humans. SIX2, containing one homeobox DNA-binding domain, is highly expressed in fetal tissues but expression is limited in adult tissues. SIX2 may be involved in limb tendon and ligament development[PMID:21420949]. It has been previously shown that SIX2 is expressed in developing mesenchymal tissue including head and urogenital system at the time of overt midfacial and renal differentiation[PMID: 22282599].

Protocols

Product Specific Protocols
FC protocol for CL594 SIX2 antibody CL594-66347Download protocol
Standard Protocols
Click here to view our Standard Protocols