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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated SKIL Polyclonal antibody

SKIL Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-19218

Synonyms

SKI like oncogene, Ski like protein, Ski related oncogene, Ski related protein, SKIL, SNO, SnoA, SnoI, SnoN

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated SKIL Polyclonal anti (CL488-19218)

    IF Staining of HepG2 using CL488-19218

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 SKIL antibody (CL488-19218) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-19218 targets SKIL in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SKIL fusion protein Ag5282 相同性解析による交差性が予測される生物種
Full Name SKI-like oncogene
Calculated molecular weight 77 kDa
Observed molecular weight 77 kDa
GenBank accession numberBC059386
Gene symbol SKIL
Gene ID (NCBI) 6498
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SKIL, also known as SnoN, is a mediator of the transforming growth factor-β (TGF-β) signaling pathway and exerts pro-oncogenic activity through inhibiting TGF-β/Smad pathway(PMID: 19114989). SKIL is involved in reduction of autophagy and subsequent tumor cell apoptosis in arsenic trioxide-treated promyelocytic leukemia and ovarian carcinoma. In addition, SKIL may regulate cell division or differentiation in response to extracellular signals.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 SKIL antibody CL488-19218Download protocol
Standard Protocols
Click here to view our Standard Protocols