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SKP2 Polyclonal antibody

Name: SKP2 Polyclonal antibody
Brand: Proteintech
SKU: 15010-1-AP
Rating: 5.00 (1 reviews)

SKP2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

Reviews (1)

Cat no : 15010-1-AP

Synonyms

F-box/LRR-repeat protein 1, F-box protein Skp2, FBL1, F box/LRR repeat protein 1, F box protein Skp2

Validation Data Gallery

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AllWBIPIHCIF/ICC

WB analysis of HEK-293 using 15010-1-AP
WB result of SKP2 antibody (15010-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-SKP2 transfected HEK-293 cells.
WB analysis using 15010-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15010-1-AP (SKP2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 15010-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 15010-1-AP (SKP2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 15010-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 15010-1-AP (SKP2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 15010-1-AP
WB result of SKP2 antibody (15010-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SKP2 transfected HEK-293 cells.
IP experiment of HEK-293 using 15010-1-AP
IP result of anti-SKP2 (IP:15010-1-AP, 4ug; Detection:15010-1-AP 1:300) with HEK-293 cells lysate 2440ug.
IHC staining of human lung cancer using 15010-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15010-1-AP (SKP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 15010-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15010-1-AP (SKP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 15010-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 15010-1-AP (SKP2 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human breast cancer using 15010-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 15010-1-AP (SKP2 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human breast cancer using 15010-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15010-1-AP (SKP2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 15010-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15010-1-AP (SKP2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 15010-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SKP2 antibody (15010-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 15010-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293 cells, Jurkat cells
Positive IP detected in	HEK-293 cells
Positive IHC detected in	human lung cancer tissue, human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:5000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 6 publications below
WB	See 44 publications below
IHC	See 8 publications below
IF	See 7 publications below
IP	See 4 publications below
CoIP	See 1 publications below

Product Information

15010-1-AP targets SKP2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SKP2 fusion protein Ag6826 相同性解析による交差性が予測される生物種
Full Name	S-phase kinase-associated protein 2 (p45)
Calculated molecular weight	48 kDa
Observed molecular weight	46-48 kDa
GenBank accession number	BC001441
Gene symbol	SKP2
Gene ID (NCBI)	6502
RRID	AB_2187647
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SKP2(S-phase kinase-associated protein 2) is also named as FBXL1 and belongs to the F-box family. It regulates cellular proliferation by targeting several cell cycle-regulated proteins for ubiquitination and degradation, including cyclin-dependent kinase inhibitor CDKN1B(PMID:22200179). The acetylation of SKP2 in the nuclear localization signal (NLS) can promote its cytoplasmic retention, and cytoplasmic SKP2 enhances cellular migration through ubiquitination and destruction of CDH. This protein has 3 isoforms produced by alternative splicing (PMID:26497688, PMID:16902410).

Protocols

Product Specific Protocols
WB protocol for SKP2 antibody 15010-1-AP	Download protocol
IHC protocol for SKP2 antibody 15010-1-AP	Download protocol
IF protocol for SKP2 antibody 15010-1-AP	Download protocol
IP protocol for SKP2 antibody 15010-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IHC,IF	Cancer Cell Hippo Signaling Suppresses Cell Ploidy and Tumorigenesis through Skp2. Authors - Shihao Zhang View Article
mouse	WB	Redox Biol Cyclic helix B peptide alleviates proinflammatory cell death and improves functional recovery after traumatic spinal cord injury Authors - Yu Xu View Article
human	WB	Nat Commun DNA damage during S-phase mediates the proliferation-quiescence decision in the subsequent G1 via p21 expression. Authors - Alexis R Barr View Article
human	WB,IHC,IF,IP	Cancer Lett SKP2 promotes the metastasis of pancreatic ductal adenocarcinoma by suppressing TRIM21-mediated PSPC1 degradation Authors - Jiahui Yuan KD Validated View Article
mouse	WB	Cell Rep The Ets transcription factor GABP is a component of the hippo pathway essential for growth and antioxidant defense. Authors - Wu Hongtan H View Article
mouse	WB	Theranostics TFE3, a potential therapeutic target for Spinal Cord Injury via augmenting autophagy flux and alleviating ER stress. Authors - Kailiang Zhou View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

Jonathan (Verified Customer) (01-11-2022)

Good clear signal with no background issues. I'm happy with the blots obtained using this antibody.

Applications: Western Blot
Primary Antibody Dilution: 1 in 1000
Cell Tissue Type: HeLa

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SKP2 Polyclonal antibody

SKP2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of HEK-293 using 15010-1-AP

WB analysis using 15010-1-AP

WB analysis of Jurkat using 15010-1-AP

WB analysis of HEK-293 using 15010-1-AP

WB analysis of HEK-293 using 15010-1-AP

IP experiment of HEK-293 using 15010-1-AP

IHC staining of human lung cancer using 15010-1-AP

IHC staining of human lung cancer using 15010-1-AP

IHC staining of human breast cancer using 15010-1-AP

IHC staining of human breast cancer using 15010-1-AP

IHC staining of human breast cancer using 15010-1-AP

IHC staining of human breast cancer using 15010-1-AP

IF Staining of HepG2 using 15010-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Hippo Signaling Suppresses Cell Ploidy and Tumorigenesis through Skp2.

Cyclic helix B peptide alleviates proinflammatory cell death and improves functional recovery after traumatic spinal cord injury

DNA damage during S-phase mediates the proliferation-quiescence decision in the subsequent G1 via p21 expression.

SKP2 promotes the metastasis of pancreatic ductal adenocarcinoma by suppressing TRIM21-mediated PSPC1 degradation

The Ets transcription factor GABP is a component of the hippo pathway essential for growth and antioxidant defense.

TFE3, a potential therapeutic target for Spinal Cord Injury via augmenting autophagy flux and alleviating ER stress.

Reviews