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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated SKP2 Polyclonal antibody

SKP2 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-15010

Synonyms

F box protein Skp2, F box/LRR repeat protein 1, FBL1, FBXL1, FLB1, p45skp2, SKP2

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated SKP2 Polyclonal anti (CL488-15010)

    IF Staining of HepG2 using CL488-15010

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 SKP2 antibody (CL488-15010) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-15010 targets SKP2 in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SKP2 fusion protein Ag6826 相同性解析による交差性が予測される生物種
Full Name S-phase kinase-associated protein 2 (p45)
Calculated molecular weight 48 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC001441
Gene symbol SKP2
Gene ID (NCBI) 6502
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SKP2(S-phase kinase-associated protein 2) is also named as FBXL1 and belongs to the F-box family. It regulates cellular proliferation by targeting several cell cycle-regulated proteins for ubiquitination and degradation, including cyclin-dependent kinase inhibitor CDKN1B(PMID:22200179). The acetylation of SKP2 in the nuclear localization signal (NLS) can promote its cytoplasmic retention, and cytoplasmic SKP2 enhances cellular migration through ubiquitination and destruction of CDH. This protein has 3 isoforms produced by alternative splicing (PMID:26497688, PMID:16902410).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 SKP2 antibody CL488-15010Download protocol
Standard Protocols
Click here to view our Standard Protocols