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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SLAM/CD150 Monoclonal antibody, PBS Only

SLAM/CD150 Monoclonal Antibody for WB, ELISA

Host / Isotype

mouse / IgM

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

5B4G1

Cat no : 60043-1-PBS

Synonyms

CD150, CDw150, IPO 3, SLAM, SLAMF1

Validation Data Gallery

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  • Western Blot (WB) analysis of Raji cells using SLAM/CD150 Monoclonal antibody (60043-1-Ig)

    WB analysis of Raji using 60043-1-Ig (same clone as 60043-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 60043-1-Ig (SLAM/CD150 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60043-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using SLAM/CD150 Monoclonal antibody (60043-1-Ig)

    WB analysis of Jurkat using 60043-1-Ig (same clone as 60043-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 60043-1-Ig (SLAM/CD150 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60043-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HUVEC cells using SLAM/CD150 Monoclonal antibody (60043-1-Ig)

    WB analysis of HUVEC using 60043-1-Ig (same clone as 60043-1-PBS)

    HUVEC cells were subjected to SDS PAGE followed by western blot with 60043-1-Ig (SLAM/CD150 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60043-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60043-1-PBS targets SLAM/CD150 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype mouse / IgM
Class Monoclonal
Type Antibody
Immunogen SLAM/CD150 fusion protein Ag1386 相同性解析による交差性が予測される生物種
Full Name signaling lymphocytic activation molecule family member 1
Calculated molecular weight 37 kDa
Observed molecular weight 80 kDa
GenBank accession numberBC012602
Gene symbol CD150
Gene ID (NCBI) 6504
Conjugate Unconjugated
Form Liquid
Purification MethodThiophilic affinity chromatograph
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

Signaling lymphocytic activation molecule (SLAM, also known as SLAMF1 or CD150) is a 70-95 kDa transmembrane glycoprotein that belongs to the immunoglobulin gene superfamily. SLAM is constitutively expressed on peripheral blood memory T-cells, T-cell clones, immature thymocytes and a proportion of B-cells, and is rapidly induced on naive T-cells after activation. SLAM is involved in T cell stimulation and cytokine production, and may play an important role in the regulation of immune responses to pathogens.