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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SLAM/CD150 Monoclonal antibody

SLAM/CD150 Monoclonal Antibody for WB, ELISA

Host / Isotype

mouse / IgM

Reactivity

human and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

5B4G1

Cat no : 60043-1-Ig

Synonyms

CD150, CDw150, IPO 3, SLAM, SLAMF1

Validation Data Gallery

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  • Western Blot (WB) analysis of Raji cells using SLAM/CD150 Monoclonal antibody (60043-1-Ig)

    WB analysis of Raji using 60043-1-Ig

    Raji cells were subjected to SDS PAGE followed by western blot with 60043-1-Ig (SLAM/CD150 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using SLAM/CD150 Monoclonal antibody (60043-1-Ig)

    WB analysis of Jurkat using 60043-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 60043-1-Ig (SLAM/CD150 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HUVEC cells using SLAM/CD150 Monoclonal antibody (60043-1-Ig)

    WB analysis of HUVEC using 60043-1-Ig

    HUVEC cells were subjected to SDS PAGE followed by western blot with 60043-1-Ig (SLAM/CD150 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inRaji cells, HUVEC cells, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60043-1-Ig targets SLAM/CD150 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity monkey
Host / Isotype mouse / IgM
Class Monoclonal
Type Antibody
Immunogen SLAM/CD150 fusion protein Ag1386 相同性解析による交差性が予測される生物種
Full Name signaling lymphocytic activation molecule family member 1
Calculated molecular weight 37 kDa
Observed molecular weight 80 kDa
GenBank accession numberBC012602
Gene symbol CD150
Gene ID (NCBI) 6504
RRIDAB_2187821
Conjugate Unconjugated
Form Liquid
Purification MethodCaprylic acid/ammonium sulfate precipitation
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Signaling lymphocytic activation molecule (SLAM, also known as SLAMF1 or CD150) is a 70-95 kDa transmembrane glycoprotein that belongs to the immunoglobulin gene superfamily. SLAM is constitutively expressed on peripheral blood memory T-cells, T-cell clones, immature thymocytes and a proportion of B-cells, and is rapidly induced on naive T-cells after activation. SLAM is involved in T cell stimulation and cytokine production, and may play an important role in the regulation of immune responses to pathogens.

Protocols

Product Specific Protocols
WB protocol for SLAM/CD150 antibody 60043-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
monkeyWB

Animals (Basel)

Signaling Lymphocytic Activation Molecule Family Member 1 Inhibits Porcine Reproductive and Respiratory Syndrome Virus Replication

Authors - Haofei Song
  • KD Validated
View Article