Validation Data Gallery
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Tested Applications
| Positive WB detected in | MJ cells, HUVEC cells, Jurkat cells, Raji cells, Daudi cells |
Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
Product Information
60043-1-Ig targets SLAM/CD150 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | monkey |
| Host / Isotype | mouse / IgM |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | SLAM/CD150 fusion protein Ag1386 相同性解析による交差性が予測される生物種 |
| Full Name | signaling lymphocytic activation molecule family member 1 |
| Calculated molecular weight | 37 kDa |
| Observed molecular weight | 80 kDa |
| GenBank accession number | BC012602 |
| Gene Symbol | CD150 |
| Gene ID (NCBI) | 6504 |
| RRID | AB_2187821 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Thiophilic affinity chromatograph |
| UNIPROT ID | Q13291 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Signaling lymphocytic activation molecule (SLAM, also known as SLAMF1 or CD150) is a 70-95 kDa transmembrane glycoprotein that belongs to the immunoglobulin gene superfamily. SLAM is constitutively expressed on peripheral blood memory T-cells, T-cell clones, immature thymocytes and a proportion of B-cells, and is rapidly induced on naive T-cells after activation. SLAM is involved in T cell stimulation and cytokine production, and may play an important role in the regulation of immune responses to pathogens.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SLAM/CD150 antibody 60043-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Animals (Basel) Signaling Lymphocytic Activation Molecule Family Member 1 Inhibits Porcine Reproductive and Respiratory Syndrome Virus Replication
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