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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using SLC12A8 Recombinant antibody (83225-2-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 83225-2-RR (same clone as 83225-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83225-2-RR (SLC12A8 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83225-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using SLC12A8 Recombinant antibody (83225-2-RR)

    IF Staining of U2OS using 83225-2-RR (same clone as 83225-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using SLC12A8 antibody (83225-2-RR, Clone: 240082B11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83225-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of MCF-7 cells using SLC12A8 Recombinant antibody (83225-2-RR)

    FC experiment of MCF-7 using 83225-2-RR (same clone as 83225-2-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug SLC12A8 Recombinant antibody (83225-2-RR, Clone:240082B11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83225-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00171-1

    Cytometric bead array standard curve of MP00171-1

    Cytometric bead array standard curve of MP00171-1, SLC12A8 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83225-1-PBS. Detection antibody: 83225-2-PBS. Standard: Ag34354. Range: 5-40 ng/mL.

  • Affinity and Kinetic Characterization of 83225-2-RR

    Affinity and Kinetic Characterization of 83225-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83225-2-RR against Human SLC12A8 were performed. The affinity constant is 3.03 nM.

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SLC12A8 Recombinant antibody, PBS Only (Detector)

SLC12A8 Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 83225-2-PBS
Clone No.240082B11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Synonyms

CCC9, Cation-chloride cotransporter 9, 240082B11

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration:  100 μg, 1mg/ml
100 μg, 1mg/ml

¥167,000/ 100 μg

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83225-2-PBS targets SLC12A8 as part of a matched antibody pair:

MP00171-1: 83225-1-PBS capture and 83225-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SLC12A8 fusion protein Ag34354 相同性解析による交差性が予測される生物種
Full Name solute carrier family 12 (potassium/chloride transporters), member 8
Calculated molecular weight 714 aa, 80 kDa
GenBank accession numberBC020506
Gene Symbol SLC12A8
Gene ID (NCBI) 84561
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDA0AV02
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.