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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SLC12A8 Recombinant antibody

SLC12A8 Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240082B11

Cat no : 83225-2-RR

Synonyms

CCC9, Cation-chloride cotransporter 9, 240082B11

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using SLC12A8 Recombinant antibody (83225-2-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 83225-2-RR

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83225-2-RR (SLC12A8 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using SLC12A8 Recombinant antibody (83225-2-RR)

    IF Staining of U2OS using 83225-2-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using SLC12A8 antibody (83225-2-RR, Clone: 240082B11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of MCF-7 cells using SLC12A8 Recombinant antibody (83225-2-RR)

    FC experiment of MCF-7 using 83225-2-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug SLC12A8 Recombinant antibody (83225-2-RR, Clone:240082B11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83225-2-RR

    Affinity and Kinetic Characterization of 83225-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83225-2-RR against Human SLC12A8 were performed. The affinity constant is 3.03 nM.

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Tested Applications

Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83225-2-RR targets SLC12A8 in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SLC12A8 fusion protein Ag34354 相同性解析による交差性が予測される生物種
Full Name solute carrier family 12 (potassium/chloride transporters), member 8
Calculated molecular weight 714 aa, 80 kDa
GenBank accession numberBC020506
Gene symbol SLC12A8
Gene ID (NCBI) 84561
RRIDAB_3670909
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for SLC12A8 antibody 83225-2-RRDownload protocol
IF protocol for SLC12A8 antibody 83225-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols