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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated SLC12A8 Recombinant antibody

SLC12A8 Recombinant Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

240082B11

Cat no : CL488-83225-2

Synonyms

CCC9, Cation-chloride cotransporter 9, 240082B11

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using CoraLite® Plus 488-conjugated SLC12A8 Recombinant (CL488-83225-2)

    IF Staining of U2OS using CL488-83225-2

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CoraLite® Plus 488 SLC12A8 antibody (CL488-83225-2, Clone: 240082B11 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of U-251 cells using CoraLite® Plus 488-conjugated SLC12A8 Recombinant (CL488-83225-2)

    FC experiment of U-251 using CL488-83225-2

    1x10^6 U-251 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated SLC12A8 Recombinant antibody (CL488-83225-2, Clone:240082B11)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inU-251 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-83225-2 targets SLC12A8 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SLC12A8 fusion protein Ag34354 相同性解析による交差性が予測される生物種
Full Name solute carrier family 12 (potassium/chloride transporters), member 8
Calculated molecular weight 714 aa, 80 kDa
GenBank accession numberBC020506
Gene symbol SLC12A8
Gene ID (NCBI) 84561
RRIDAB_3673223
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 SLC12A8 antibody CL488-83225-2Download protocol
FC protocol for CL Plus 488 SLC12A8 antibody CL488-83225-2Download protocol
Standard Protocols
Click here to view our Standard Protocols