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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SLC14A2 Polyclonal antibody (21932-1-AP)

    WB analysis using 21932-1-AP (same clone as 21932-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21932-1-AP (SLC14A2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21932-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using SLC14A2 Polyclonal antibody (21932-1-AP)

    WB analysis using 21932-1-AP (same clone as 21932-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21932-1-AP (SLC14A2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21932-1-PBS in a different storage buffer formulation.

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SLC14A2 Polyclonal antibody, PBS Only

SLC14A2 Polyclonal Antibody for WB, Indirect ELISA
Cat No. 21932-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21932-1-PBS targets SLC14A2 in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLC14A2 fusion protein Ag16561 相同性解析による交差性が予測される生物種
Full Name solute carrier family 14 (urea transporter), member 2
Calculated molecular weight 920 aa, 101 kDa
Observed molecular weight43 kDa, 101 kDa
GenBank accession numberBC110445
Gene Symbol SLC14A2
Gene ID (NCBI) 8170
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15849
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

SLC14A2, also known as human urea transporter -2 (HUT2), is the human homolog of UT-A2 and the only kidney-specific urea transporter cloned in humans. Urea transport in the kidney is mediated by a family of transporter proteins, including renal urea transporters (UT-A) and erythrocyte urea transporters (UT-B). UT-A proteins expressed in the kidney play a critical role in generating the hypertonic medulla, which is an integral component of the urinary concentrating mechanism (PMID: 11590132, 11880324). The 4.2-kb hUT-A1 cDNA encodes a 920-amino acid peptide, which is 89% identical to the rat UT-A1 protein (PMID: 11502588).