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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using SLC20A1 Polyclonal antibody (12423-1-AP)

    WB analysis of HEK-293 using 12423-1-AP (same clone as 12423-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12423-1-AP (SLC20A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12423-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using SLC20A1 Polyclonal antibody (12423-1-AP)

    WB analysis of K-562 using 12423-1-AP (same clone as 12423-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 12423-1-AP (SLC20A1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12423-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using SLC20A1 Polyclonal antibody (12423-1-AP)

    WB analysis of Jurkat using 12423-1-AP (same clone as 12423-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 12423-1-AP (SLC20A1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12423-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using SLC20A1 Polyclonal antibody (12423-1-AP)

    WB analysis of HeLa using 12423-1-AP (same clone as 12423-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 12423-1-AP (SLC20A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12423-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human kidney tissue using SLC20A1 Polyclonal antibody (12423-1-AP)

    WB analysis of human kidney using 12423-1-AP (same clone as 12423-1-PBS)

    human kidney tissue were subjected to SDS PAGE followed by western blot with 12423-1-AP (SLC20A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12423-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using SLC20A1 Polyclonal antibody (12423-1-AP)

    IP experiment of HEK-293 using 12423-1-AP (same clone as 12423-1-PBS)

    IP result of anti-SLC20A1 (IP:12423-1-AP, 4ug; Detection:12423-1-AP 1:500) with HEK-293 cells lysate 2800ug. This data was developed using the same antibody clone with 12423-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using SLC20A1 Polyclonal antibody (12423-1-AP)

    IHC staining of human liver using 12423-1-AP (same clone as 12423-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12423-1-AP (SLC20A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12423-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using SLC20A1 Polyclonal antibody (12423-1-AP)

    IHC staining of human liver using 12423-1-AP (same clone as 12423-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12423-1-AP (SLC20A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12423-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SLC20A1 Polyclonal antibody (12423-1-AP)

    IHC staining of human breast cancer using 12423-1-AP (same clone as 12423-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12423-1-AP (SLC20A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12423-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SLC20A1 Polyclonal antibody (12423-1-AP)

    IHC staining of human breast cancer using 12423-1-AP (same clone as 12423-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12423-1-AP (SLC20A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12423-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using SLC20A1 Polyclonal antibody (12423-1-AP)

    IHC staining of human lymphoma using 12423-1-AP (same clone as 12423-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 12423-1-AP (SLC20A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12423-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using SLC20A1 Polyclonal antibody (12423-1-AP)

    IHC staining of human lymphoma using 12423-1-AP (same clone as 12423-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 12423-1-AP (SLC20A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12423-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

SLC20A1 Polyclonal antibody, PBS Only

SLC20A1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 12423-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12423-1-PBS targets SLC20A1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLC20A1 fusion protein Ag3084 相同性解析による交差性が予測される生物種
Full Name solute carrier family 20 (phosphate transporter), member 1
Calculated molecular weight 679 aa, 74 kDa
Observed molecular weight 85 kDa
GenBank accession numberBC019944
Gene Symbol SLC20A1
Gene ID (NCBI) 6574
RRIDAB_2286212
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WUM9
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

SLC20A1 (also known as PiT1) is a high-affinity sodium-dependent phosphate (Pi) import protein. It is widely expressed and plays a fundamental housekeeping role in phosphate transport, such as absorbing phosphate from interstitial fluid for normal cellular functions. SLC20A1 is involved in cell proliferation, tumor growth and cell signaling independently of its transport function. SLC20A1 also functions as a retroviral receptor, causing human cells to be susceptible to infection by gibbon ape leukemia virus, simian sarcoma-associated virus, feline leukemia virus subgroup B, and 10A1 murine leukemia virus.