CoraLite® Plus 488-conjugated SLC25A1 Monoclonal antibody
SLC25A1 Monoclonal Antibody for WB
Host / Isotype
Mouse / IgG3
Reactivity
human, mouse, rat
Applications
WB
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1F11A3
Cat no : CL488-66771
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-66771 targets SLC25A1 in WB applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG3 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | SLC25A1 fusion protein Ag7520 相同性解析による交差性が予測される生物種 |
| Full Name | solute carrier family 25 (mitochondrial carrier; citrate transporter), member 1 |
| Calculated molecular weight | 34 kDa |
| Observed molecular weight | 34 kDa |
| GenBank accession number | BC004980 |
| Gene symbol | SLC25A1 |
| Gene ID (NCBI) | 6576 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SLC25A1, also known as CIC or CTP, is a mitochondrial citrate transporter that exports citrate from the mitochondria to the cytosol. SLC25A1 is highly expressed in several tumor types. Recently it has been identified as a novel transcriptional target of mutant p53 and a negative tumor prognostic marker. In addition, defects in SLC25A1 has been found as a cause of combined D-2- and L-2-hydroxyglutaric aciduria. This antibody specifically recognized the endogenous SLC25A1; no protein was detected in cells from subjects containing two alleles with truncating mutations with this antibody. (24681808, 23561848)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 488 SLC25A1 antibody CL488-66771 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
