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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CoraLite® Plus 488-conjugated SLC25A1 Monoclonal a (CL488-66771)

    WB analysis using CL488-66771

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-66771 (SLC25A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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CoraLite® Plus 488-conjugated SLC25A1 Monoclonal antibody

SLC25A1 Monoclonal Antibody for WB
Cat No. CL488-66771
Clone No.1F11A3

Host / Isotype

Mouse / IgG3

Reactivity

human, mouse, rat

Applications

WB

Synonyms

Tricarboxylate carrier protein, SLC20A3, CTP, Citrate transport protein, CIC

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66771 targets SLC25A1 in WB applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Immunogen SLC25A1 fusion protein Ag7520 相同性解析による交差性が予測される生物種
Full Name solute carrier family 25 (mitochondrial carrier; citrate transporter), member 1
Calculated molecular weight 34 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC004980
Gene Symbol SLC25A1
Gene ID (NCBI) 6576
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP53007
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SLC25A1, also known as CIC or CTP, is a mitochondrial citrate transporter that exports citrate from the mitochondria to the cytosol. SLC25A1 is highly expressed in several tumor types. Recently it has been identified as a novel transcriptional target of mutant p53 and a negative tumor prognostic marker. In addition, defects in SLC25A1 has been found as a cause of combined D-2- and L-2-hydroxyglutaric aciduria. This antibody specifically recognized the endogenous SLC25A1; no protein was detected in cells from subjects containing two alleles with truncating mutations with this antibody. (24681808, 23561848)

Protocols

Product Specific Protocols
WB protocol for CL Plus 488 SLC25A1 antibody CL488-66771Download protocol
Standard Protocols
Click here to view our Standard Protocols