CoraLite® Plus 750-conjugated SLC25A5 Recombinant antibody
SLC25A5 Recombinant Antibody for WB
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB
Conjugate
CoraLite® Plus 750 Fluorescent Dye
CloneNo.
230247B1
Cat no : CL750-83104
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | C6 cells, rat liver tissue, mouse liver tissue, Neuro-2a cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL750-83104 targets SLC25A5 in WB applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | SLC25A5 fusion protein Ag11956 相同性解析による交差性が予測される生物種 |
| Full Name | solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 5 |
| Calculated molecular weight | 298 aa, 33 kDa |
| Observed molecular weight | 32 kDa |
| GenBank accession number | BC056160 |
| Gene symbol | SLC25A5 |
| Gene ID (NCBI) | 292 |
| Conjugate | CoraLite® Plus 750 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 755 nm / 780 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Adenine nucleotide translocators (ANTs) are mitochondrial carrier proteins that exchange ATP and ADP between the cytoplasm and the mitochondrial matrix. Four isoforms of ANT were identified: ANT1 (encoded by SLC25A4) is highly expressed in different tissues; ANT2 (encoded by SLC25A5) is predominantly expressed in proliferating, regenerating, or undifferentiated cells; ANT3 is ubiquitously expressed; ANT4 is mainly expressed in testis and germ cells. This antibody recognizes both of ANT1 and ANT2. The predicted molecular weight of ANT1/2 protein is around 32 kDa. While 60 kDa protein can also be observed, which has been proposed as the dimer of ANT1/2. (22423112)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 750 SLC25A5 antibody CL750-83104 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
