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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 750-conjugated SLC25A5 Recombinant antibody

SLC25A5 Recombinant Antibody for WB

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

230247B1

Cat no : CL750-83104

Synonyms

ANT1/2, ADP/ATP translocase 2, N-terminally processed, ADP/ATP translocase 2, ADP,ATP carrier protein 2, AAC2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CoraLite® Plus 750-conjugated SLC25A5 Recombinant (CL750-83104)

    WB analysis using CL750-83104

    Various lysates were subjected to SDS PAGE followed by western blot with CL750-83104 (SLC25A5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of PC-3 cells using CoraLite® Plus 750-conjugated SLC25A5 Recombinant (CL750-83104)

    FC experiment of PC-3 using CL750-83104

    1x10^6 PC-3 cells were intracellularly stained with 0.4 ug CoraLite® Plus 750-conjugated SLC25A5 Recombinant antibody (CL750-83104, Clone:230247B1)(red), or 0.4 ug Isotype Control (CL750-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inC6 cells, rat liver tissue, mouse liver tissue, Neuro-2a cells
Positive FC (Intra) detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL750-83104 targets SLC25A5 in WB applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SLC25A5 fusion protein Ag11956 相同性解析による交差性が予測される生物種
Full Name solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 5
Calculated molecular weight 298 aa, 33 kDa
Observed molecular weight32 kDa
GenBank accession numberBC056160
Gene symbol SLC25A5
Gene ID (NCBI) 292
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission maxima wavelengths755 nm / 780 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Adenine nucleotide translocators (ANTs) are mitochondrial carrier proteins that exchange ATP and ADP between the cytoplasm and the mitochondrial matrix. Four isoforms of ANT were identified: ANT1 (encoded by SLC25A4) is highly expressed in different tissues; ANT2 (encoded by SLC25A5) is predominantly expressed in proliferating, regenerating, or undifferentiated cells; ANT3 is ubiquitously expressed; ANT4 is mainly expressed in testis and germ cells. This antibody recognizes both of ANT1 and ANT2. The predicted molecular weight of ANT1/2 protein is around 32 kDa. While 60 kDa protein can also be observed, which has been proposed as the dimer of ANT1/2. (22423112)

Protocols

Product Specific Protocols
WB protocol for CL Plus 750 SLC25A5 antibody CL750-83104Download protocol
Standard Protocols
Click here to view our Standard Protocols