Validation Data Gallery
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Tested Applications
| Positive WB detected in | HepG2 cells, HeLa cells |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human kidney tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 16 publications below |
| IHC | See 3 publications below |
| IF | See 6 publications below |
Product Information
19429-1-AP targets ZIP7 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ZIP7 fusion protein Ag13762 相同性解析による交差性が予測される生物種 |
| Full Name | solute carrier family 39 (zinc transporter), member 7 |
| Calculated molecular weight | 469 aa, 50 kDa |
| Observed molecular weight | 45-50 kDa, 56 kDa |
| GenBank accession number | BC000645 |
| Gene Symbol | ZIP7 |
| Gene ID (NCBI) | 7922 |
| RRID | AB_10643376 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q92504 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ZIP7 is a functional zinc transporter transporting zinc from the Golgi apparatus to the cytoplasm of the cell. ZIP7 is post-translationally regulated by CK2-mediated phosphorylation. This ZIP7 phosphorylation results in zinc release from intracellular stores, which activates multiple tyrosine kinases and regulate cell survival and proliferation. Dual bands of 50 kDa and 56 kDa detected by this antibody may represent the native and phosphorylated forms of ZIP7, respectively. (PMID: 28232492, 28205653)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ZIP7 antibody 19429-1-AP | Download protocol |
| IHC protocol for ZIP7 antibody 19429-1-AP | Download protocol |
| IF protocol for ZIP7 antibody 19429-1-AP | Download protocol |
| IP protocol for ZIP7 antibody 19429-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Chem Sci X-ray fluorescence imaging reveals subcellular biometal disturbances in a childhood neurodegenerative disorder. | ||
Cell Death Differ Systematic genetic mapping of necroptosis identifies SLC39A7 as modulator of death receptor trafficking. | ||
Acta Neuropathol Commun Deregulation of subcellular biometal homeostasis through loss of the metal transporter, Zip7, in a childhood neurodegenerative disorder.
| ||
Metallomics Deregulation of biometal homeostasis: the missing link for neuronal ceroid lipofuscinoses? | ||