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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated ZIP7 Polyclonal antibody

ZIP7 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-19429

Synonyms

D6S115E, D6S2244E, H2 KE4, HKE4, KE4, RING5, SLC39A7, Zinc transporter SLC39A7, ZIP7

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using CoraLite® Plus 488-conjugated ZIP7 Polyclonal anti (CL488-19429)

    IF Staining of HEK-293 using CL488-19429

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite® Plus 488 ZIP7 antibody (CL488-19429) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-19429 targets ZIP7 in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZIP7 fusion protein Ag13762 相同性解析による交差性が予測される生物種
Full Name solute carrier family 39 (zinc transporter), member 7
Calculated molecular weight 469 aa, 50 kDa
Observed molecular weight 45-50 kDa, 56 kDa
GenBank accession numberBC000645
Gene symbol ZIP7
Gene ID (NCBI) 7922
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ZIP7 is a functional zinc transporter transporting zinc from the Golgi apparatus to the cytoplasm of the cell. ZIP7 is post-translationally regulated by CK2-mediated phosphorylation. This ZIP7 phosphorylation results in zinc release from intracellular stores, which activates multiple tyrosine kinases and regulate cell survival and proliferation. Dual bands of 50 kDa and 56 kDa detected by this antibody may represent the native and phosphorylated forms of ZIP7, respectively. (PMID: 28232492, 28205653)

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 ZIP7 antibody CL488-19429Download protocol
Standard Protocols
Click here to view our Standard Protocols