Validation Data Gallery
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Tested Applications
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-19429 targets ZIP7 in IF/ICC applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ZIP7 fusion protein Ag13762 相同性解析による交差性が予測される生物種 |
| Full Name | solute carrier family 39 (zinc transporter), member 7 |
| Calculated molecular weight | 469 aa, 50 kDa |
| Observed molecular weight | 45-50 kDa, 56 kDa |
| GenBank accession number | BC000645 |
| Gene Symbol | ZIP7 |
| Gene ID (NCBI) | 7922 |
| RRID | AB_3672706 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q92504 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ZIP7 is a functional zinc transporter transporting zinc from the Golgi apparatus to the cytoplasm of the cell. ZIP7 is post-translationally regulated by CK2-mediated phosphorylation. This ZIP7 phosphorylation results in zinc release from intracellular stores, which activates multiple tyrosine kinases and regulate cell survival and proliferation. Dual bands of 50 kDa and 56 kDa detected by this antibody may represent the native and phosphorylated forms of ZIP7, respectively. (PMID: 28232492, 28205653)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 ZIP7 antibody CL488-19429 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |