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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SLC5A8 Polyclonal antibody (29827-1-AP)

    WB analysis using 29827-1-AP (same clone as 29827-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29827-1-AP (SLC5A8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29827-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using SLC5A8 Polyclonal antibody (29827-1-AP)

    IHC staining of rat brain using 29827-1-AP (same clone as 29827-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 29827-1-AP (SLC5A8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29827-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using SLC5A8 Polyclonal antibody (29827-1-AP)

    IHC staining of rat brain using 29827-1-AP (same clone as 29827-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 29827-1-AP (SLC5A8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29827-1-PBS in a different storage buffer formulation.

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SLC5A8 Polyclonal antibody, PBS Only

SLC5A8 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 29827-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Sodium-coupled monocarboxylate transporter 1, Sodium iodide-related cotransporter, SMCT1, SMCT, Apical iodide transporter

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

29827-1-PBS targets SLC5A8 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLC5A8 fusion protein Ag30183 相同性解析による交差性が予測される生物種
Full Name solute carrier family 5 (iodide transporter), member 8
Calculated molecular weight 610 aa, 67 kDa
Observed molecular weight 67 kDa
GenBank accession numberBC110492
Gene Symbol SLC5A8
Gene ID (NCBI) 160728
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N695
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

The sodium-coupled monocarboxylate transporter 1 (SMCT1; SLC5A8), belonging to the SLC5A family, is a 67-kDa protein that functions as a Na+-coupled electrogenic transporter for SCFAs. SLC5A mRNA and protein are expressed in healthy human, rat, and mouse colon. The protein is localized to the apical membrane of the colonocytes. Immunohistochemical studies revealed the selective localization of SMCT1 on the brush border in the villi of the terminal ileum and crypts of the colon in mice.