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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SLC5A8 Polyclonal antibody, PBS Only

SLC5A8 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 29827-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SLC5A8 Polyclonal antibody (29827-1-AP)

    WB analysis using 29827-1-AP (same clone as 29827-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29827-1-AP (SLC5A8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29827-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using SLC5A8 Polyclonal antibody (29827-1-AP)

    IHC staining of rat brain using 29827-1-AP (same clone as 29827-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 29827-1-AP (SLC5A8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29827-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using SLC5A8 Polyclonal antibody (29827-1-AP)

    IHC staining of rat brain using 29827-1-AP (same clone as 29827-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 29827-1-AP (SLC5A8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29827-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

29827-1-PBS targets SLC5A8 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLC5A8 fusion protein Ag30183 相同性解析による交差性が予測される生物種
Full Name solute carrier family 5 (iodide transporter), member 8
Calculated molecular weight 610 aa, 67 kDa
Observed molecular weight 67 kDa
GenBank accession numberBC110492
Gene symbol SLC5A8
Gene ID (NCBI) 160728
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The sodium-coupled monocarboxylate transporter 1 (SMCT1; SLC5A8), belonging to the SLC5A family, is a 69-kDa protein functions as a Na+-coupled electrogenic transporter for SCFAs. SLC5A mRNA and protein are expressed in the healthy human, rat, and mouse colon. And the protein is localized to apical membrane of the colonocytes. Immunohistochemical studies revealed the selective localization of SMCT1 on the brush border in the villi of the terminal ileum and crypts of the colon in mice.